糖尿病大鼠肠道、胰腺GLP-1受体mRNA的表达  

Expression of GLP-1 receptor mRNA in intestine and pancreas of diabetic rats

作　　者：陈榕[1] 王莉[1] 曹宏伟[1] 汤朝武[2] 白小智[2] 姬秋和[1] 

机构地区：[1]第四军医大学西京医院内分泌科,陕西西安710033 [2]第四军医大学西京医院烧伤科,陕西西安710033

出　　处：《第四军医大学学报》2008年第13期1235-1238,共4页Journal of the Fourth Military Medical University

摘　　要：目的:研究胰高血糖素样多肽1(GLP-1)受体mRNA表达水平在大鼠肠道不同部位和胰腺分布的特点及两者间的差异.方法:以Wistar大鼠为对照,口服葡萄糖耐量试验确定自发性2型糖尿病模型GK大鼠已发病,采用实时定量PCR方法(SYBR Green法)检测大鼠不同肠段和胰腺的GLP-1受体mRNA表达水平.结果:GLP-1受体mRNA表达中,正常大鼠不同肠段以空肠表达量最低,与回肠和结肠相比较差异有统计学意义(P<0.05),回肠与结肠相比较差异无统计学意义(P>0.05).糖尿病组大鼠空肠GLP-1受体mRNA相对表达量为0.27,而对照组大鼠空肠的相对表达量为0.59;糖尿病组大鼠结肠GLP-1受体mRNA相对表达量为0.5,对照组大鼠结肠的相对表达量为1;糖尿病组大鼠胰腺GLP-1受体mRNA相对表达量为0.37,而对照组大鼠胰腺的相对表达量为0.97,两组间空肠、结肠和胰腺GLP-1受体mRNA相对表达量差异均有统计学意义(P<0.05).糖尿病组回肠GLP-1受体mRNA相对表达量为0.73,对照组回肠的相对表达量为0.86,两组间差异无统计学意义(P>0.05).结论:糖尿病大鼠GLP-1受体mRNA相对表达量在空肠、结肠和胰腺的下调,提示空肠、结肠和胰腺GLP-1受体的表达变化可能与GK大鼠的糖尿病发生发展有关.AIM： To observe the distribution and difference of the expression of glucagon like peptide-1 （ GLP-1 ） receptor mRNA in different parts of intestine and pancreas in rats. METHODS： Four Wistar rats were enrolled into control group. Oral glucose tolerance test was used to identify the spontaneous type 2 diabetic GK rat models. Real-time quantitative PCR （SYBR Green ） was done to detect the expression of GLP-1 receptor mRNA in different parts of intestine and pancreas in rats. RESULTS ： The jejunum had the lowest expression level of GLP-1 receptor mRNA compared with ileum and colon in normal rats （P 〈 0.05 ） , and there was no difference in GLP-1 receptor mRNA expression level between ileum and colon （ P 〉 0.05 ）. Relative expression quantity of GLP-1 receptor mRNA in jejunum of diabetic rats was 0.27, while it was 0.59 in control group; GLP-1 receptor mRNA relative expression quantity in colon of diabetic group was 0.5, while it was 1 in control group; the relative expression quantity in pancreas of diabetic group was 0.37, while 0.97 in control group ; the differences of GLP-1 receptor mRNA expression were statistically significant between the 2 groups in jejunum, colon and pancreas （P 〈 0.05 ）. GLP-1 receptor mRNA relative expression quantity in ileum of diabetic group was 0.73, while the quantity was 0.86 in control group, with no statistical difference between the 2 groups （ P 〉 0. 05 ）. CONCLUSION： The downregulation of GLP-1 receptor mRNA expression in the jejunum, colon and pancreas in diabetic rats suggests that the changes in the expression of GLP-1 receptor mRNA in jejunum, colon and pancreas may be related to the development of diabetes in GK rat.

关 键 词：受体 胰高血糖素 GK大鼠 实时定量PCR 

分 类 号：R825.8[医药卫生—临床医学]