含T_3冷晶体停搏液对大鼠心功能及冠脉内皮细胞功能的影响  

Effect of Triiodothyronine in Cold Crystalloid Cardioplegia on Cardiac Function and the Function of Coronary Endothelial Cells

作　　者：朱亚彬[1] 顾懋栋[1] 吴熹[1] 马旺扣[1] 

机构地区：[1]南京铁道医学院附属医院胸心外科

出　　处：《南京铁道医学院学报》1997年第4期249-251,共3页Journal of Nanjing Railway Medical College

摘　　要：目的：观察冷晶体停搏液中加入生理浓度Ｔ３对大鼠停搏心肌收缩及舒张功能恢复、心肌酶谱、氧耗量及冠脉内皮细胞功能的影响。方法：运用离体鼠心作功模型，实验组（ｎ＝６）含０．６ｍｇ／ＬＴ３，比较停搏９０ｍｉｎ前后左室收缩压力（ＬＶＳＰ）、左室收缩压力微分（ＬＶ＋ｄｐ／ｄｔｍａｘ）、左室舒张压力微分（ＬＶ－ｄｐ／ｄｔｍａｘ）、肌酸磷酸激酶（ＣＰＫ）、乳酸脱氢酶（ＬＤＨ）的变化。同时评价乙酰胆碱（Ａｃｈ）和硝普钠（ＳＮＰ）所致的冠脉血管舒张功能的改变。结果：实验组ＬＶＳＰ恢复率为（９０．１±３．７）％，ＬＶ＋ｄｐ／ｄｔｍａｘ为（７９．２±５４）％，优于对照组；而ＬＶ－ｄｐ／ｄｔｍａｘ、ＬＤＨ、ＣＰＫ、Ｖｍｏ２（即ＭＶＯ２）两组无显著差异。Ａｃｈ所致的冠状血管阻力（ＣＶＲ）降低两组都不明显，ＳＮＰ所致ＣＶＲ下降程度基本一致。结论：含Ｔ３冷晶体停搏液仅能促进大鼠左室收缩功能恢复，而对舒张功能、心肌氧耗量、心肌酶谱影响不大，Ｔ３由于内皮细胞功能降低而不能明显扩张冠状血管。bjective:The effect of triiodothyronine at physiological concentration in cold crystalloid cardioplegia on the cardiac function recovery,myocardial enzymogram,oxygen consumption and coronary vascular endothelial function after 90-minute arrest was evaluated.Method:Twelve isolated rat hearts were devided into 2 groups(n=6) with cardioplegia containing 0.6mg/LT3 in experimental group.The isolated working rat heart model was used to measure left ventricular systolic pressure(LVSP),left ventricular+dp/dtmax(LV+dp/dtmax),creatine phosphokinase(CPK) and lactic dehydrogenase(LDH).The acetylcholine-(Ach-) or sodium nitroprusside-SNP induced coronary vasodilation was also measured.Results:Experimental group had a significantly higher recovery of LVSP and LV+dp/dtmax than placebo group did [(90.1±3.7)% vs(66.2±2.1)%;(79.2±5.4)% vs (64.2±6.7)% P<0.05],respectively.No difference in CPK,LDH,LV-dp/dtmax and Vmo2 was found between two groups.T3 had no effect on Ach-or SNP-induced vasodilation.Conclusion:T3 in cold crystalloid cardioplegia improves LVSP and LV+dp/dtmax recovery and exerts few influence on heart diastolic function,Vmo2 and myocardiac enzymogram.It can't reduce coronary vascular resistance because of the injury or endothelium.

关 键 词：心功能 内皮细胞 大鼠 T3 心肌酶谱 氧耗量 

分 类 号：R654.1[医药卫生—外科学]