镧示踪检测低功率毫米波辐射对HL60细胞膜通透性的影响(英文)  

Effect of low power millimeter wave irradiation on HL60 cellular membrane permeability by lanthanum tracing detection

作　　者：赖晃文[1] 江悦华[1,2] 王捷[3] 赖声礼[1] 杨传红[3] 吴小丽[3] 张宏斌[3] 

机构地区：[1]华南理工大学电子信息工程学院 [2]解放军广州军区广州总医院医学实验中心广东省广州市510010 [3]解放军广州军区广州总医院医学实验中心

出　　处：《中国组织工程研究与临床康复》2008年第26期5177-5180,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助项目(60071033);广东省自然科学基金资助项目(990587);广东省科技社会发展项目(2002C31101)~~

摘　　要：学术背景:尽管一些实验已提出细胞膜可能是电磁生物效应的靶细胞部位之一的证据,但揭示电磁辐射细胞膜的通透性以及认识电磁辐射细胞生物效应的报道仍较少。目的:探讨低功率毫米波辐射与人白血病HL60细胞膜通透性及细胞凋亡的关系。设计:辐射组4组与非辐射组对照实验。单位:实验于2006-11/2007-04在解放军广州军区广州总医院医学实验中心实验室完成。材料:人白血病HL60细胞株由解放军广州军区医学实验中心提供,HD-413.2HPSG100毫米波辐射装置由西安恒达微波技术开发公司研制。方法:应用频率为41.32GHz、平均功率密度为2mW/cm2的毫米波辐射HL60细胞,根据辐射时间分为辐射0,15,30,45,60min组。主要观察指标:镧示踪法观察各组细胞内外镧颗粒分布,评价细胞膜通透性变性的变化;透射电镜观察超微结构细胞形态特点;原位标记法行细胞凋亡阳性定量分析。结果:①毫米波辐射HL60细胞45min和60min可见少量镧颗粒进入细胞质内,超微结构观察显示线粒体肿胀,粗面内质网扩张,细胞凋亡易见。②TUNEL法染色显示辐射15,30,45,60min组凋亡率较0min组有增加的趋势,尤以45min和60min组细胞凋亡明显增加。结论:低功率毫米波辐射HL60细胞,导致细胞膜通透性增加是促进细胞凋亡及产生系列生物学效应的重要因素之一。BACKGROUND： There are some studies proposing cell membrane may be one of target cell regions for electromagnetic biological effects. However, reports responsible for cellular membrane permeability and cellular biological effects after electromagnetic irradiation are few, OBJECTIVE： This study was designed to investigate the correlations of low-power millimeter wave irradiation to cellular membrane permeability and apoptosis of HL60, DESIGN： Controlled experiment, SETTING： Thus study was performed at the Medical Experimental Central Laboratory, Guangzhou General Hospital, Guangzhou Military Area Command of Chinese PLA between November 2006 and April 2007, MATERIALS： The human leukemic cell lines HL60 were kindly provided by Medical Experimental Center of Guangzhou Military Area Command of Chinese PLA and HD-413.2HPSG100 millimeter wave irradiation generator was developed by Xi＇an Hengda Microwave Technology Development Company, China. METHODS： HL60 cells were irradiated by millimeter wave at frequency of 41.32 GHz and mean power density of 2 mW/cm^2, and divided into five groups according to the irradiation time （0, 15, 30, 45, 60 minutes groups）. MAIN OUTCOME MEASURES： Lanthanum tracing was used to observe intracellular and extracellular lanthanum particles distribution and evaluate the change of cellular membrane permeability; ultrastructure and morphological characteristics of HL60 cells were observed through an transmission electron microscope; Quantitative analysis of apoptotic cells was performed by in situ marking method, RESULTS： In the 45 and 60 minutes groups, a small amount of lanthanum particles in the cytoplasm, swollen mitochondria, expanded rough endoplasmic reticulum, and obvious apoptosis were detected by ultrastructure observation, TUNEL staining showed, compared with the 0 minute control group, the apoptosis rate showed a trend of elevation in all the irradiation groups, particularly in the 45 and 60 minutes groups. CONCLUSION： Low-power millimeter wave irradiation on HL6

关 键 词：毫米波 HL60细胞 镧示踪 细胞膜通透性 细胞凋亡 

分 类 号：R445.2[医药卫生—影像医学与核医学]