甲状旁腺激素对人成骨样细胞膜型基质金属蛋白酶1表达的调控  

Regulation of membrane-type matrix metalloproteinase-1 expression in human osteoblast-like MG-63 cells by parathyroid hormone

作　　者：李继斌[1,2] 谢辉[1] 袁凌清[1] 罗湘杭[1] 

机构地区：[1]中南大学湘雅二医院代谢内分泌研究所,湖南省长沙市410911 [2]湖南省双峰县人民医院内分泌科,湖南省娄底市417700

出　　处：《中国组织工程研究与临床康复》2008年第28期5406-5409,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:甲状旁腺激素是骨代谢的重要调节因子,膜型基质金属蛋白酶1在骨吸收的调控中起重要作用。目的:观察甲状旁腺激素对人成骨样MG-63细胞膜型基质金属蛋白酶1表达的影响。设计、时间及地点:观察对比实验,于2001-03/2002-07在中南大学湘雅二医院代谢内分泌研究所实验室完成。材料:人成骨样MG-63细胞株,甲状旁腺激素(1～34)。方法:分别应用1,10,100nmol/L的甲状旁腺激素(1～34)干预MG-63细胞24h;或分别用10nmol/L甲状旁腺激素(1～34)干预MG-63细胞2,6,24,48h。每干预组设3个平行样本,重复3次。主要观察指标:采用Northern杂交法检测膜型基质金属蛋白酶1 mRNA表达水平;Western杂交法检测膜型基质金属蛋白酶1蛋白表达水平。结果:①1,10,100nmol/L的甲状旁腺激素(1～34)对MG-63细胞膜型基质金属蛋白酶1 mRNA及蛋白的表达有抑制作用,呈剂量依赖性(P<0.05)。②10nmol/L的甲状旁腺激素(1～34)在干预2～48h内对膜型基质金属蛋白酶1 mRNA及蛋白的表达有抑制作用,呈时间依赖性(P<0.05)。结论:甲状旁腺激素具有抑制成骨样细胞膜型基质金属蛋白酶1表达的作用,此可能为甲状旁腺激素诱导骨吸收的作用途径之一。BACKGROUND： Parathyroid hormone is an important regulatory factor in the bone metabolism, and membrane-type matrix metalloproteinase-1 plays an important role in the regulation of bone resorption. OBJECTIVE： To observe the effects of parathyroid hormone on the expression of membrane-type matrix metalloproteinase-1 in human osteoblast-like MG-63 cells. DESIGN, TIME AND SETTING： Contrast observation was conducted at the laboratory of Institute of Endocrinology ＆ Metabolism, the Second Xiangya Hospital of Central South University between March 2001 and July 2002. MATERIALS： Human osteoblast-like MG-63 cell line; parathyroid hormone （1-34）. METHODS： MG-63 cells were treated with 1, 10, 100 nmol/L parathyroid hormone （1-34） for 24 hours, respectively, or were treated with 10 nmol/L parathyroid hormone （1-34） for 2, 6, 24, 48 hours, respectively. Each intervention group had three parallel samples and each intervention was repeated for three times. MAIN OUTCOME MEASURES： Northern Blotting were used to determine the expression level of membrane-type matrix metalloproteinase-1 mRNA; Western Blotting were performed to detect the protein expression of membrane-type matrix metalloproteinase-1. RESULTS： Our study showed that 1, 10, 100 nmol/L parathyroid hormone （1 34） inhibited membrane-type matrix metalloproteinase-1 mRNA and protein expression in a dose-dependent manner in MG-63 cells （P 〈 0.05）. Within 2-48 hours of intervention, 10 nmol/L parathyroid hormone （1-34） inhibited membrane-type matrix metaLloproteinase-1 mRNA and protein expression in a time-dependent manner in MG-63 cells （P 〈 0.05）. CONCLUSION： Parathyroid hormone can inhibit the expression of membrane-type matrix metalloproteinase-1 in osteoblast-like MG-63 ceils, it may be one of the ways with which parathyroid hormone induces bone resorption.

关 键 词：甲状旁腺激素类 金属蛋白酶类 成骨细胞 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]