膝骨关节炎患者血清基质金属蛋白酶3、肿瘤坏死因子α、白细胞介素1、透明质酸、脂质过氧化物含量及超氧化物歧化酶活性变化与补肾活血中药的干预  

Effects of compound herb of invigorating kidney and promoting blood flow on serum matrix metalloproteinases-3,tumor necrosis factor alpha,interleukin-1,hyaluronic acid,lipid peroxidation levels and superoxide dismutase activity in patients with knee osteo

作　　者：曾意荣[1] 樊粤光[1] 吴凡[1] 李显澎[1] 范海蛟[1] 

机构地区：[1]广州中医药大学第一附属医院髋中心,广东省广州市510405

出　　处：《中国组织工程研究与临床康复》2008年第28期5436-5439,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:补肾活血复方中药能改善膝骨关节炎患者的症状,但对其作用途径尚无统一认识。目的:观察补肾活血复方中药对膝骨关节炎患者血清中基质金属蛋白酶3、肿瘤坏死因子α、白细胞介素1、透明质酸、脂质过氧化物含量及超氧化物歧化酶活性的影响。设计、时间及地点:病例-对照及自身对照的细胞因子及酶学检测实验,于2002-09/2005-01在广州中医药大学第一附属医院髋中心完成。对象:选择广州中医药大学第一附属医院门诊或病房收治的膝骨关节炎患者42例,按随机数字表法分为2组(n=21),即补肾活血中药组和骨仙片组。另外选择10例无关节疾病的骨折愈合后来本院拆除内固定的患者作为正常对照组。方法:补肾活血中药组以补肾活血方治疗,中药复方由杜仲、熟地、枸杞子、补骨脂、川牛膝、独活、红花、木瓜、川芎、丹参和木香组成,由广州中医药大学第一附属医院自制,5片/次,3次/d;骨仙片组采用口服中成药骨仙片治疗,4片/次,3次/d。30d为1个疗程,共治疗1个疗程。主要观察指标:补肾活血中药组和骨仙片组治疗前及治疗1个疗程后,分别采用酶联免疫法、四甲基偶氮唑蓝法、双抗体夹心酶联免疫吸附实验及放射免疫法测定患者血清基质金属蛋白酶3、肿瘤坏死因子α、白细胞介素1及透明质酸含量;应用试剂盒测定血清脂质过氧化物含量及超氧化物歧化酶活性,并与正常对照组对比。结果:治疗前补肾活血中药组和骨仙片组患者血清基质金属蛋白酶3、白细胞介素1及透明质酸含量均高于正常对照组(P<0.01)。治疗1个疗程后,补肾活血中药组和骨仙片组患者血清基质金属蛋白酶3、白细胞介素1、透明质酸及脂质过氧化物含量均低于治疗前(P<0.05～0.01),血清超氧化物歧化酶活性高于治疗前(P<0.05)。结论:补肾活血中药与骨仙片具有类似的效果,可降低膝骨关节炎患BACKGROUND： Compound herb of invigorating kidney and promoting blood flow can improve knee osteoarthritis, but its action mechanism is still unclear. OBJECTIVE： To investigate the effects of compound herb of invigorating kidney and promoting blood flow on serum matrix metalloproteinases （MMP）-3, tumor necrosis factor （TNF）-α , interleukin-1, hyaluronic acid, lipid peroxidation and superoxide dismutase （SOD） levels in patients with knee osteoarthritis. DESIGN, TIME AND SETTING： The case-control and own control experiments of cytokines and enzymology determination were performed at the Hip Center, First Affiliated Hospital, Guangzhou University of Chinese Medicine from September 2002 to January 2005. PARTICIPANTS： Totally 42 patients with knee osteoarthritis were enrolled at the Out-patient clinic or Ward of First Affiliated Hospital, Guangzhou University of Chinese Medicine. They were randomized into 2 groups （n=21）, an experimental group and a control group. Ten patients without joint diseases, who received internal fixation removal after fracture union, were enrolled as normal controls. METHODS： Patients in the experimental group underwent compound herb of invigorating kidney and promoting blood flow. The compound herb was composed of eucommia bark, prepared rhizome of rehmannia, barbary wolfberry fruit, psoralea fruits, cyathula officinalis kuan, doubleteeth pubescent angelica root, fits carthami, papaya, szechwan lovge rhizome and salvia mildorrhiza and costustroot. The compound herb was made by Guangzhou University of Chinese Medicine, China. Patients took once five pills, three times a day. Patients in the control group were orally administrated with Guxian tablet, once four tablets, three times a day. Thirty days were a course, totally one course. MAIN OUTCOME MEASURES： Before treatment, and one course after treatment, serum MMP-3, TNF-α, interleukin-1 and hyalnronic acid in patients from the experimental and control groups were respectively detected by enzyme-linked

关 键 词：骨关节炎 膝/中药疗法 补肾 活血 金属蛋白酶类 肿瘤坏死因子 白细胞介素1 透明质酸 超氧化物歧化酶 过氧化脂质类 

分 类 号：R684.3[医药卫生—骨科学]