丹参酮ⅡA对鼠脑缺血再灌注损伤MCP-1和IL-1β含量的影响  

Effect of Tanshinone ⅡA on the Contents of MCP-1 and IL-1βfollowing Cerebral Ischmia Reperfusion Injury in Rats

作　　者：李浩[1] 刘开祥[1] 俸军林[1] 

机构地区：[1]桂林医学院附属医院,桂林541002

出　　处：《中国中医急症》2008年第7期957-958,975,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：广西自然科学基金资助项目(No.0542110)

摘　　要：目的探讨丹参酮ⅡA(TanⅡA)对局灶性脑缺血再灌注损伤大鼠脑组织单核细胞趋化蛋白-1(MCP-1)和白细胞介素-1β(IL-1β)含量的影响。方法将大鼠随机分为假手术组、缺血再灌注组、TanⅡA低剂量治疗组和TanⅡA高剂量治疗组,线栓法建立局灶性脑缺血再灌注模型。TanⅡA高、低剂量治疗组分别于术前连续灌胃给予高、低剂量TanⅡA3d,每日1次。各组于脑缺血90min再灌注24h进行2,3,5-三苯基氯化四氮唑染色观察脑梗死体积和脑含水量检测,采用酶联免疫吸附试验法检测脑组织MCP-1和IL-1β含量的变化。结果TanⅡA治疗组脑梗死体积较缺血再灌注组减小,脑含水量较缺血再灌注组减少,而高、低剂量组之间差异亦具有显著性。与假手术组比较,缺血再灌注组脑组织MCP-1和IL-1β含量明显升高;与缺血再灌注组比较,TanⅡA高、低剂量治疗组脑组织MCP-l和IL-1β含量均明显降低,高、低剂量组之间差异具有显著性。结论降低缺血再灌注损伤脑组织MCP-1和IL-1β含量,抑制再灌注损伤炎症反应,可能是TanⅡA发挥脑保护的重要途径之一。Objective:To study effect of Tanshinone ⅡA(Tan ⅡA) on the contents of MCP-1 and IL-1βfollowing cerebral ischemia reperfusion (I/R) injury in rats.Methods:Rats were randomly divided into 4 groups,which were sham operated gorup,I/R group,low dose Tan ⅡA treatment gorup and high dose Tan ⅡA treatment group.The focal middle cerebral arterymocclusion (MCAO) model was made by suture-occluded method.Rats were pretreated with Tan ⅡA ,ig for 3d,respectively befroe MCAO.After 90min MCAO following 24h of reperfusion,TTC staining and brain water content was investigated.The contents of MCP-1 and IL-1βwas also investigated with ELISA.Results:Compard with that of I/R group, the cerebral infarction volume and water content of low and high dose Tan ⅡA treatment group reduced. Compared with sham operated group,the contents of MCP-1 and IL-1βincreased obviously.Compared with I/R group,low and high dose Tan ⅡA treatment group reduced the contents of MPC-1 and IL-1βdose-dependently.Conclusion:Tan ⅡA may reduced cerebral ischemia-reperfusion injure by reducing the contents of MCP-1 and IL-1βdose-dependently.It plays protective effect on cerebral ischemia injury.

关 键 词：缺血再灌注 单核细胞趋化蛋白-1 白细胞介素-1B 丹参酮ⅡA 

分 类 号：R285.5[医药卫生—中药学]