三抗杨叶片高效再生系统的建立  

High Efficiency Regeneration from in vitro Leaf of Populus 'Sankang'

作　　者：温安娜[1] 郑彩霞[1] 杨明嘉[1] 徐佳[1] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083

出　　处：《山西林业科技》2008年第2期1-4,共4页Shanxi Forestry Science and Technology

基　　金：北京林业大学研究生自选课题基金资助(06jj062)

摘　　要：以三抗杨为试材,建立了三抗杨叶片外植体高效再生系统,为三抗杨遗传转化体系的建立奠定了良好的基础。以三抗杨无菌苗叶片作外植体,接种于附加不同浓度的生长素(NAA,IAA)和细胞分裂素(6-BA)的MS培养基上,筛选出叶片分化不定芽的最佳培养基为MS+6-BA 1.0 mg/L+NAA 0.1 mg/L,蔗糖30 g/L,琼脂6.0 g/L,pH值5.8,不定芽分化率为83.3%。将不定芽接种于附加不同浓度的生长素(NAA,IBA)的MS培养基上,筛选出三抗杨最佳生根培养基为MS+IBA 0.1 mg/L+NAA 0.1 mg/L,蔗糖15 g/L,琼脂6.0 g/L,pH值5.8,生根率为91.7%。Regeneration system of Populus ＇Sankang＇ has been established from leaf explants, which laid the foundation for the establishment of genetic transformation system. In vitro leaves of Populus ＇Sankang＇ were cultured as explants on MS medium containing various combinations of growth regulators. It was shown that the best medium for differentiation of adventitious buds was MS medium supplemented with 1.0 mg/L 6-BA, 0.1 mg/L NAA, 30 g/L sucrose and 6 g/L agar, PHS. 8. Under such circumstances, the regeneration rate was 83.3 %. As compared with other mediums, the most suitable rooting medium was MS medium supplemented with 0.1 mg/L IBA, 0.1 mg/L NAA, 15 g/L sucrose and 6 g/L agar,pHS. 8. And the rooting rate reached 91.7 %.

关 键 词：三抗杨 叶片外植体 再生系统 

分 类 号：S722.37[农业科学—林木遗传育种]