骨髓细胞移植改善宿主肝细胞功能研究  

Improvement of host liver cell function by bone marrow cells transplantation

作　　者：张彪[1] 姜波健[1] 夏焱[1] 郑元超[1] 稻恒光裕 葛西真一 

机构地区：[1]上海交通大学医学院附属第三人民医院普外科,201900 [2]日本旭川医科大学第二外科

出　　处：《中华实验外科杂志》2008年第7期863-865,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察骨髓间充质细胞（BMCs）移植能否提高严重肝功能损害合并再生障碍的同种先天性无白蛋白大鼠（F344alb）肝再生和修复能力。方法F344大鼠为供体，F344alb受体鼠接受Retrorsine（RS）1次／2周腹腔注射2次后4周行2／3肝切除（PH）。正常F344alb为组I（n=5）；BMCs移植为组Ⅱ（n=8）；RS／PH预处理为组Ⅲ（n=8）；RS／PH预处理后BMCs移植为组Ⅳ（n=8）；RS／PH预处理后肝实质细胞移植为组Ⅴ（n=8）。4周后行各组大鼠肝脏形态学和组化染色研究，检测肝功能，及肝组织和骨髓基因检测。结果（1）生存率：组IV75％，组V50％，组Ⅲ37．5％，组Ⅰ和组Ⅱ100％。（2）4周后组Ⅴ肝再生率（67．38±8．66）％显著高于组Ⅳ、Ⅲ[（55．31±8．69）％，（44．27±6．51）％]。（3）PH后1d，组Ⅲ、Ⅳ、Ⅴ血清TB、ALT显著升高；PH后2d，组Ⅳ血清TB、ALT显著下降。（4）组Ⅳ、Ⅴ肝组织切片白蛋白免疫组织化学染色显示白蛋白染色阳性肝细胞呈簇状分布。（5）F344来源白蛋白基因片段出现在组Ⅳ、Ⅴ大鼠肝组织内。（6）PH后2、4周，组Ⅳ、Ⅴ血清白蛋白显著升高。结论BMCs移植可提高严重肝损合并再生障碍受体鼠肝再生能力，保护肝功能，促进肝修复。Objective To investigate whether bone marrow cells （BMCs） transplantation could promote the restoration of the injured liver in Nagase＇ s analbuminemic rats. Methods Fischer 344 rats （F344） and congenic Nagase＇ s analbuminemic rats （F344alb） were respectively used as donors and recipients. F344alb received pyrrolizidine alkaloid retrorsine （RS） （ 30 mg/kg, i. p. ） two times, once 2 weeks. Four weeks later, 1 × 10^7 BMCs or 2 × 106 hepatocytes from F344 were transplanted into portal vein immediately after 2/3 partial hepatectomy （PH）. F344alb rats were divided into following groups： neither RS/PH treatment nor cells transplantation （Group I , n = 5 ） ;BMCs transplantation alone （Group Ⅱ ,n = 8 ） ; RS/PH treatment alone （Group Ⅲ, n = 8 ） ;BMCs transplantation with RS/PH treatment （Group Ⅳ, n = 8） ;hepatocytes transplantation with RS/PH treatment （Group Ⅴ, n = 8 ）. Morphological, histochemical and serological examinations for recipients＇ liver function and serum albumin were performed post-PH. The normal albumin gene was detected respectively from recipients＇ liver and bone marrow. Results Survival rate was significantly increased by BMCs transplantation, but not increased by hepatocytes transplantation. Both BMCs and hepatocytes transplantation stimulated liver regeneration. TB and ALT were significantly increased in rats of groups Ill and V at day 1,2 post-PH. BMCs transplantation could protect the liver function after being damaged by RS-exposed and PH. Clusters of hepatocytes were detected in livers of groups IV and V at 4th week post-PH. Normal albumin gene sequence was detected in the liver of groups IV and V. Serum albumin levels were increased in groups IV and ／ . Conclusion F344-derived BMCs transplantation could promote the restoration of the injured liver in F344alb, and increase the survival rate in serious liver-damaged.

关 键 词：移植 骨髓细胞 肝细胞 白蛋白 

分 类 号：R657.3[医药卫生—外科学]