GRGDS修饰D，L-丙交酯-β-苹果酸共聚物的生物相容性研究  

BIOCOMPATIBILITY STUDIES ON GRGDS SEQUENCE MODIFIED POLY(D,L-LACTIDE-co-β-MALIC ACID)

作　　者：刘媛[1] 王珺[1] 王蔚[1] 贾小华[1] 袁直[1] 俞耀庭[2] 

机构地区：[1]功能高分子材料教育部重点实验室南开大学高分子所,天津300071 [2]南开大学生命科学学院生物活性材料教育部重点实验室,天津300071

出　　处：《高分子学报》2008年第7期635-638,共4页Acta Polymerica Sinica

基　　金：国家重点基础研究发展计划(973计划,项目号2005CB623904);武汉大学生物医用高分子材料教育部重点实验室开放课题基金(基金号20070404)资助项目

摘　　要：聚(D,L-丙交酯-β-苹果酸)(P-COOH)进行改性制得GRGDS修饰聚合物(P-GS5),利用红外光谱、元素分析和X射线光电子能谱确定了材料的结构和组成.通过内皮细胞粘附实验发现P-GS5可明显提高内皮细胞粘附(PDLLA,45%;P-GS5,109%),改善材料的细胞亲和性;同时血小板粘附实验结果也表明P-GS5可有效抑制血小板粘附(PDLLA,47%;P-GS5,18%),降低血栓形成的几率.A novel peptide-grafted polymer P-GS5 was synthesized by modification of poly（D,L-lactide-co-β-malic acid） （P-COOH） with pentapeptides Gly-Arg-Gly-Asp-Ser （GRGDS）, and its biocompatibility was investigated. Infrared spectroscopy （IR）, element analysis （EA） and X-ray photoelectron spectroscopy （XPS） were used to characterize the synthesized materials （poly （ D, L-lactide） （PDLLA）, P-COOH, P-GS5） , Hydrophilic properties of these polymers were investigated by contact angle measurements （CA） . Subsequently, biocompatibility experiments were conducted by testing human umbilical cord vein endothelial cell （HUVEC） （24 h） and platelet （30 min） adhesion on polymer surfaces, The number of endothelial cell and platelet adhesion was counted by cytometer and automatic hematological analyzer, respectively. It has been shown that the hydrophilicity of P-GS5 was greatly improved. Water contact angle was 84° for PDLLA,78° for P-COOH and 58° for P-GS5. Cell adhesion data indicated that P-GS5 could promote endothelial cell attachments. The adhesion ratio of PDLLA, P-COOH and P-GS5 was 45% ,62% and 109%, respectively. According to the platelet adhesion results, GRGDS on P-GS5 did not induce platelet aggregation,The platelet adhesion capacity of P-GS5 was much lower than that of the other two polymers, while the adhesion ratio of PDLLA, P-COOH and P-GS5 was 47%,37 % and 18%, respectively. In conclusion, P-GS5 displayed improved hydrophilicity,higher affinity to endothelial cell and better ability of anti-platelet adhesion compared with PDLLA and P-COOH. It could be a potential biomedical polymer.

关 键 词：抗血小板粘附 GRGDS D L-丙交酯-β-苹果酸共聚物 

分 类 号：R318.08[医药卫生—生物医学工程]