胶束体系中荧光素与四溴荧光素间能量转移条件及模型研究  

Study on the Mechanism of Energy Transfer between Fluorescein Sodium and Tetrabromofluorescein Sodium in Micelles of Cationic Surface Active Agent

作　　者：刘保生[1] 张彦青[1] 王云科[1] 吕运开[1] 

机构地区：[1]河北大学理化分析中心,河北省分析科学技术重点实验室,河北保定071002

出　　处：《光谱学与光谱分析》2008年第7期1600-1602,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(20075005)资助

摘　　要：研究了阳离子表面活性剂(CSAA)在水溶液中与阴离子酸性染料四溴荧光素(TBF)的荧光反应,发现当CSAA单体与TBF形成离子缔合物时,TBF的荧光发生猝灭,而CSAA胶束与TBF作用又会产生一个新的、更强的荧光。进而研究了阳离子表面活性剂CTMAB及CPB胶束体系中酸性阴离子荧光染料荧光素与四溴荧光素间的能量转移条件。表明只有在带相反电荷的CSAA形成的胶束中,阴离子染料间的能量转移才可能发生,在2/3临界胶束浓度(CMC)时能量转移效率达到最大。并推测了胶束体系中染料间能量转移模型及染料间能量转移的一般规律。In the present paper, the fluorescence reaction of cationic surface-active agents （CSAA） with Tetrabromofluorescein sodium （TBF） in aqueous solution was investigated. It was found that the fluorescence quenching of TBF appears when it reacts with the cation monomer of a CSAA and a new stronger fluorescence is obtained when the ion-associates react with the micellate of CSAA. The authors investigated the condition of energy transfer between acidic fluorescent dyes in micelles of cetyl trimethyl ammonium bromide or hexadeeyl pyridinium bromide. It was indicated that in the mieelles formed by cationic surface active agent with dyes embedded （cationic surface active agent and dyes are charge opposite）, the effective energy transfer between anionic dyes could occur. When the concentration of cationic surface active agent reached certain value, the energy transfer could occur. With the value of two thirds of critical micelle concentration, the efficiency of energy transfer reached the maximum; when the concentration of cationic surface active agent increased, the efficiency of energy transfer would be decreased. The authors also deduced the model of energy transfer between dyes in micelles and laws of it.

关 键 词：阴离子表面活性剂 能量转移 荧光素 四溴荧光素 模型 

分 类 号：O657.3[理学—分析化学]