微波辅助萃取-分光光度法测定卫矛中总黄酮  

Determination of Total Flavones from Euonymus Alatus Sieb by Microwave-Assisted Extraction and Absorption Spectroscopy

作　　者：张帆[1] 周峰[1] 王桂花[1] 杨屹[1] 郭振库[2] 

机构地区：[1]北京化工大学理学院,北京100029 [2]北京瑞利分析仪器公司,北京100016

出　　处：《光谱学与光谱分析》2008年第7期1633-1636,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(20375004)资助

摘　　要：采用微波辅助萃取法提取，以分光光度法测定卫矛样品中的总黄酮含量。并且通过单因素实验和正交实验设计优化了溶剂浓度、溶剂用量、微波功率和微波辐射时间等微波提取条件，确定了微波辅助萃取法提取卫矛中黄酮类化合物的最佳条件为：使用60％乙醇，在液固比40：1条件下，以255W的微波功率，微波辐射5min。在上述最优条件下进行了精密度和回收率实验，所得结果的相对标准偏差为1．71％（n=5），回收率在97％～101％之间。与传统的索氏提取法和超声波提取法比较，微波辅助萃取法具有操作简便，经济可靠，重现性好等特点。该实验采用的微波辅助萃取-分光光度法测定法简便高效，可推广应用于各类中草药中总黄酮的含量测定。Determination of total flavones from euonymus alatus sieb by microwave-assisted extraction and absorption spectroscopy was reported. The effects of solvent concentration, ratio of solution to solid, microwave power and radiation time were evaluated by single factor and orthogonal experiments. The extraction conditions of flavones from euonymus alatus sieb by microwave-assisted method were optimized as 40 ： 1 of the weight ratio of 60% （V/V） ethanol to twigs and microwave power of 255 W for 5 min. Under the optimized conditions the relative standard deviation is 1.71% （n=5） and the recovery is 97%-101%. Compared with Soxhlet extraction and ultrasound-assisted extraction, microwave-assisted extraction was simple and accurate with good reproducibility. It is suitable for the determination of flavone in many kinds of traditional Chinese medicines.

关 键 词：微波辅助萃取 分光光度法 卫矛 黄酮 

分 类 号：O657.3[理学—分析化学]