窄冠白杨组培快繁与再生体系的建立  

Tissue Culture and Regeneration System from Leaves of Populus Leucopyramidalis in vitro

作　　者：李际红[1] 邢世岩[1] 焦振[1] 刘国兴[1] 张友朋[1] 

机构地区：[1]山东农业大学林学院,泰安271018

出　　处：《中国农学通报》2008年第7期60-64,共5页Chinese Agricultural Science Bulletin

基　　金：国家重点推广项目"窄冠黑杨与窄冠黑白杨推广项目"(2003EC000219)

摘　　要：以窄冠白杨为试材,对其组培快繁与再生体系的建立进行了初步的研究。研究表明,抗氧化剂聚乙烯吡咯烷酮(PVP)和抗坏血酸(Vc)能有效降低外植体的褐化;该品种外植体增殖的最适培养基为1/2MS+BA0.3mg/L+NAA0.05mg/L,平均分化率为87%,增殖系数达7.5倍,与其它处理组合均呈极显著差异。硝普钠(SNP)12mg/L极显著地促进叶片不定芽分化,其再生频率与再生芽个数分别达到96.5%与8.2。窄冠白杨适宜的生根培养基为1/2MS+IBA0.3mg/L。In this report, tissue culture and regeneration system from leaves in vitro have been extensively investigated utilizing Populus Leucopyramidalis as experiment materials. The results showed that the anti-oxidation materials polyvinylpyrrolidone （PVP） and ascorbic acid （Vc） decreased browning efficiently; the best medium for proliferation was 1/2 MS＋ BA0.3mg/L ＋ NAA 0.05mg/L with differentiation percentage of 87% and proliferation multiples of 7.5 respectively, and there was a great significant difference compared with other treatments. The differentiation and regeneration of adventitious shoot from leaves increased significantly in the medium MS containing BA0.3mg/L and NAA0.05mg/L supplemented with 12mg/L sodium nitroprusside （SNP） in which regeneration frequency and average number of shoots were 100% and 12 respectively. The best rooting medium was 1/2 MS＋ IBA0.3mg/L .

关 键 词：窄冠白杨 组培快繁 再生体系 硝普钠 

分 类 号：S722.37[农业科学—林木遗传育种]