牛杀菌/通透性增加蛋白氮端基因的克隆和序列分析  

Cloning and Sequence Analyzing of Bovine Bactericidal/permeability-increasing Protein N-terminal Fragment

作　　者：高恒[1] 彭开松[1] 祁克宗[1] 江龙海[1] 

机构地区：[1]安徽农业大学动物科技学院,合肥230036

出　　处：《中国奶牛》2008年第7期8-10,共3页China Dairy Cattle

基　　金：安徽省教育厅自然科学研究项目(KJ2007A021)

摘　　要：本试验应用RT-PCR技术,参照Genbank报道的序列,从荷斯坦牛中性粒细胞mRNA中扩增出杀菌/通透性增加蛋白(BPI)氮端基因(713bp),并与pGEM-T-easy载体连接,构建基因重组体pGEM-T-easy-BPI,进行序列测定。结果表明获得长度为713bp的BPI氮端基因。序列分析证实该片段与安哥斯牛BPI氮端基因相比有1个点突变,为同义突变。该基因的克隆为进一步表达该基因奠定了基础。The study aimed to clone cDNA of N-terminal bovine bactericidal/permeability-increasing protein（BPI）and to construct the recombinant plasmid pGEM-T-easy-BPL According to the sequence in Genbank, the gene which encodes N-terminal BPI protein was amplified by RT-PCR from mRNA that were extracted from the bovine polymorphonuclear neutrophils （PMN）.The PCR product was cloned into the pGEM-T-easy vector and the sequence was confirmed by sequencing. The result showed that a 713bp gene was acquired. Compared with the sequence in Genbank, there is a silent mutation of one base pair.

关 键 词：杀菌/通透性增加蛋白 牛 序列测定 

分 类 号：S823.2[农业科学—畜牧学]