热胁迫对耐热性不同黄瓜品种及其F1代叶片膜透性和保护酶活性的影响  

Effects of heat stress on the protective enzyme activities and membrane permeabilities in the leaves of seedlings of cucumber varieties and their F_1 hybrid

作　　者：李为观[1] 杨寅桂[1] 江彪[1] 娄丽娜[1] 陈劲枫[1] 

机构地区：[1]作物遗传与种质创新国家重点实验室.南京农业大学园艺学院,南京210095

出　　处：《中国瓜菜》2008年第2期1-5,共5页China Cucurbits And Vegetables

基　　金：国家自然科学基金资助项目(30470120,30671419);国家“863”项目(2006AA10Z1A8,2006AA100108);博士点基金(20050307009);国家支撑计划(2006BAD01A7-5-11);江苏省高新技术研究计划(BG2007301)

摘　　要：为了选育耐热黄瓜品种,以黄瓜(Cucumis sativus L.)耐热品种Poinsett97、热敏品种Boothbyls Blonde和它们的F1代为试验材料,研究黄瓜幼苗在28、38℃条件下,叶片中保护性酶类活性和膜透性的变化。结果表明:在38℃热胁迫下,耐热品种的相对电导率、MDA含量提高量明显低于热敏品种,而其保护酶SOD、POD活性和PRO含量的提高量明显高于热敏品种,CAT活性的降低量明显低于热敏品种。在F1代中,POD活性表现正向杂种优势外,其余均为负向优势。Cucumber varieties Poinsett 97, a heat tolerance variety, Boothbyls Blonde, a heat susceptive variety, and their F1 Polnsett 97×Boothbyls Blonde were used to research the dynamic changes of the activities of membrane protective enzymes （superoxide dismutase -SOD, peroxidase-POD, catalase-CAT ）, the content of malondialdehyde （ MDA ）, the content of PRO and relative conductance （RC） of the leaves under 28 ℃ and 38 ℃. The results showed that under heat stress the increase of RC, MDA content was evidently lower;the increase of the SOD, POD activity and the content of PRO was evidently higher. The decrease of CAT activity was evidently lower in the heat tolerance variety than the heat susceptive variety. The positive heterosis of POD was observed, negative heterosis of the others index were found in the F1.

关 键 词：黄瓜 热胁迫 保护酶 膜透性 杂种优势 

分 类 号：S642.2[农业科学—蔬菜学]