绿僵菌几丁质酶活性及其对椰心叶甲毒力的相关性分析  

Extracellular chitinase activities of Metarhizium anisopliae and its correlation with toxicity of Brontispa longissima

作　　者：石晓珍[1] 王敏[1] 黄华平[1] 郭立佳[1] 宋妍[1] 黄俊生[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所//海南省热带农业有害生物检测监控重点实验室,海南儋州571737

出　　处：《广西农业科学》2008年第3期313-316,共4页Guangxi Agricultural Sciences

基　　金：国家科技基础条件平台工作项目(2005DKA30506);科技基础工作和社会公益研究专项(2004DIB3J073)

摘　　要：用液体培养法和琼脂平板透明圈法研究了6个绿僵菌菌株的几丁质酶产酶水平与其对椰心叶甲毒力的关系。结果显示，各菌株的毒力大小顺序为JF813E〉JF86C、JF883〉Ma4〉JF83G〉JF842；对椰心叶甲成虫的LT50值在6、3～8．0d之间；液体培养法所测酶活变化范围为5．9～38．4IU／mL，琼脂平板法所得比值在1．37～2．43之间。表明不同绿僵菌菌株的产酶水平存在明显差异，且产几丁质酶水平的高低与其对椰心叶甲的毒力有一定的相关性；几丁质酶活性可以作为一个参考指标对绿僵菌菌种进行初步筛选，但不能完全代替毒力测定而成为菌种质量性状的检测指标。The extracellular chitinase of 6 Metarhizium anisopliae and its correlation with the toxicity of Brontispa longissima by the culture method of shaking flask and agar-flat were analyzed. The results showed that the toxicity of strains could be ranked as follows： JF813E 〉 JF86C, JF883 〉 Ma4 〉 JF83G 〉 JF842. LTs0 of Brontispa longissima larvae was between 6.3- 8.0d. The range of enzyme activities determined by shaking flask culture method and agar-flat method was 5.9-38.4 IU/mL and 1.37-2.43 U/mL, respectively. It indicated that the enzyme production ability in different strains of Metarhizium anisopliae had obvious difference, and the ability had a certain of correlation with the toxicity of Brontispa longissirna. The chitinase activities could be used as a reference indicator to preliminary screen the strains, but it couldn＇t totally substitute the toxicity test.

关 键 词：金龟子绿僵菌 几丁质酶 椰心叶甲 毒力测定 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]