多发性硬化患者外周血单个核细胞转录因子Sp3基因表达缺失及其与免疫的关系  

Deficient expression of Sp3 gene in peripheral blood mononuclear cells in multiple sclerosis patients and the correlation with immunity

作　　者：张留福[1] 田淑芬[1] 梁金[1] 郭明升[1] 楚兰[1] 

机构地区：[1]贵阳医学院附属医院神经内科,550004

出　　处：《中华神经科杂志》2008年第7期437-439,共3页Chinese Journal of Neurology

摘　　要：目的 研究多发性硬化（MS）患者外周血单个核细胞（PBMC）转录因子Sp3基因的表达情况及其与机体免疫功能的关系。方法 贵州地区发病的汉族MS患者31例及健康对照30名，采用逆转录PCR（RT-PCR）检测其PBMC的Sp3基因表达情况；双抗体夹心ELISA法检测血清可溶性自细胞介素-2受体（sIL-2R）水平。结果 MS组与健康对照组PBMC cDNA扩增Sp3基因阴性率差异有统计学意义[分别为41．9％（12／31）和6．7％（2／30），Х^2=7．133，P=0．008]。MS组血清sIL-2R含量较健康对照组明显升高[（2788．5±1079．8）、（1270．6±489．4）μg／L，t=6．170，P=0．001]，其中Sp3基因阴性[（3364．0±1252．3）μg／L]、阳性[（2450．0±827．0）μg／L]表达患者血清sIL-2R含量较健康对照组均明显升高（F=32．059，P〈0．05），Sp3基因阴性表达患者比阳性表达患者sIL-2R含量明显升高（q=4．213，P〈0．05）。结论贵州地区发病的汉族MS患者PBMC中存在Sp3基因表达缺失，提示汉族MS患者Sp3基因表达无明显的地域差异。sIL-2R参与了MS的发病过程，Sp3基因表达缺失后机体发生了更严重的免疫功能障碍。Objective To evaluate the expression of Sp3 gene of peripheral blood mononuclear cells （PBMC） in multiple sclerosis （MS） patients in Guizhou and the relationship between Sp3 gene expression and immunological function. Methods Two pairs of primers were used to amplify cDNAs generated from 31 MS patients and 30 healthy controls. The serum levels of sIL-2R were measured in 27 patients with MS and 30 healthy controls by sandwiched ELISA. Results The deficient expression of Sp3 gene in MS patients was significantly higher than that in control （41.9% （12/31） vs 6.7% （2/30）,Х^2 = 7. 133, P = 0. 008 ）. The sIL-2R levels in MS patients were significantly higher than those in control （ 2788, 5 ± 1079. 8 ）, （ 1270. 7 ±489.4 ） μg/L, t = 6. 170, P = 0. 001 ） . The concentration of sIL-2R in MS with negative （ （3364. 0 ± 1252. 3 ） μg/L ） and positive （ （ 2450.0 ± 827. 0 ） μg/L ） expression of Sp3 gene were significantly increased compared with control （ F = 32. 059, P 〈 0. 05 ）. The slL-2R levels were significantly rising in MS patients with negative expression of Sp3 gene compared with MS patients with positive expression of Sp3 gene （ q = 4. 213, P 〈 0.05 ）. Conclusions A remarkable deficient expression of Sp3 gene in PBMC has been found in MS patients in Guizhou. slL-2R may take part in the process of MS. The expression of Sp3 gene is not affected by immune state, however, MS patients with Sp3 deficient expression tend to have a more serious impairment in immunological functions.

关 键 词：多发性硬化 白细胞 单核 Sp3转录因子 基因表达 受体 白细胞介素2 

分 类 号：R686[医药卫生—骨科学]