柴芩承气汤对急性胰腺炎大鼠腺泡细胞外分泌功能的影响  

Effects of ChaiQinChengQi Decoction on Exocrine Function of Pancreatic Acinar cells of Acute Pancreatitis Rats

作　　者：邓力珲[1] 杨晓楠[1] 黄蕾[1] 向大开[1] 夏庆[1] 

机构地区：[1]四川大学华西医院中西医结合科,成都610041

出　　处：《四川大学学报（医学版）》2008年第4期555-557,566,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30500684);国家中医药管理局课题基金(批准号04-05JP55)资助

摘　　要：目的研究柴芩承气汤(CQCQ-D)对急性胰腺炎(AP)大鼠胰腺腺泡细胞外分泌功能和腺泡细胞内钙离子浓度([Ca2+]i)变化的影响。方法SD大鼠灌喂CQCQ-D以制备柴芩承气汤含药血清(CQCQ-S);SD大鼠分为AP组和假手术组,分离胰腺腺泡细胞并与CQCQ-S共同孵育,观测腺泡细胞淀粉酶分泌水平和[Ca2+]i变化。结果AP大鼠腺泡细胞淀粉酶分泌水平较假手术组降低(P<0.01),CQCQ-S使AP大鼠腺泡细胞淀粉酶分泌水平进一步降低(P<0.05);[Ca2+]i随AP病程延长而升高(P<0.05),CQCQ-S可抑制AP大鼠腺泡细胞内[Ca2+]i升高(P<0.05)。结论CQCQ-D对AP大鼠的胰腺腺泡细胞的外分泌功能和腺泡细胞内钙超载有抑制作用。Objective To investigate the effects of Chaiqinchengqi decoction（CQCQ-D）on exocrine function of pancreatic acinar cells and the changes of intracellular calcium concentration（[Ca^2＋]i）of acute pancreatitis（AP）rats,so as to explore the mechanism of CQCQ-D.Methods Acute pancreatitis model was induced in Sprague-Dawley rat by ligation of bile-pancreatic duct,pancreatic acinar cells were isolated by collagenase digestion and in vitro co-incubated with the drug serum containing Chaiqingchengqi decoction（CQCQ-S）which was collected from normal rats intragastrically fed on CQCQ-D,then the amylase activity secreted into the buffer was assayed and intracellular fluorescent intensity were observed and analyzed to investigate the changes of intracellular calcium concentration（[Ca^2＋]i）.Results Amylase secretion in AP group was lower than that in sham-operation（SO）group（P〈0.01）,while CQCQ-S treatment led to more significant decrease（P〈0.05）;[Ca^2＋]i elevated in AP rats（P〈0.05）,while the level of [Ca^2＋]i elevation was redu ced after CQCQ-S treatment（P〈0.05）.Conclusion CQCQ-D has inhibitive effects on exocrine function of pancreatic acinar cells and the calcium overload of AP rats.

关 键 词：急性胰腺炎 柴芩承气汤 腺泡细胞 外分泌 钙超载 

分 类 号：R285.5[医药卫生—中药学]