乌司他丁对大鼠肾脏机械性损伤后肾脏HSP70、IL-6表达的早期影响  

Ulinastatin's Early Effects on Kidney's Expression of HSP70 and IL-6 after Mechanical Injury of Kidney

作　　者：毛渭东[1] 孙少华[1] 

机构地区：[1]兰州大学第一医院病理研究所,兰州730000

出　　处：《四川大学学报（医学版）》2008年第4期591-594,共4页Journal of Sichuan University(Medical Sciences)

摘　　要：目的探讨乌司他丁在大鼠肾脏机械性损伤后对肾脏组织IL-6和HSP70表达的影响及意义。方法66只Wistar大鼠,随机分为非创伤对照组、单纯创伤组、创伤后给予乌司他丁组。采用自由落体生物撞击仪撞击大鼠脊肋角复制创伤动物模型。用组织芯片技术及免疫组织化学方法检测各组创伤后1h、6h、12h、24h、36h肾脏组织中IL-6和HSP70蛋白的表达。结果对照组肾脏组织中IL-6和HSP70蛋白均呈弱阳性表达。与对照组比较,单纯创伤组在创伤后1、6、12、24h点IL-6表达增强(P<0.05),6h点HSP70表达增强(P<0.05)。创伤后注射乌司他丁组各时相点肾组织中IL-6表达明显低于单纯创伤组(P<0.05),HSP70表达在1h、24h、36h点明显高于单纯创伤组(以1h点最为显著,P<0.05)。结论乌司他丁可能通过抑制IL-6的过度表达,上调HSP70的表达,改变两者表达的比例,在创伤早期(以1h点为著)对大鼠肾脏起保护性作用。Objective To investigate the significance of ulinastatin＇s effects on kidney＇s expression of IL-6,HSP70 after mechanical injury.Methods Sixity-six adult female Wistar rats were randomly divided into three groups：6 in the group of control,30 in the group of trauma,30 in the group injected ulinastatin after trauma.The model of trauma rats was made with free-fall bio-impactor striking the spinal column and ribs of rats.To detect the IL-6 and HSP70 expression of each group＇s renal tissue at 1 h,6 h,12 h,24 h and 36 h after trauma with tissue chip and immunohistochemical stain.Results IL-6 and HSP70 expression of renal tissue was low with group of control.Compared with group of control,the expression of IL-6 strengthened at 1 h,6 h,12 h,24 h after trauma（P〈0.05）,the expression of HSP70 with group of trauma strengthened at 6 h after trauma（P〈0.05）.The expression of IL-6 with group injected ulinastatin after trauma were more lower than group of trauma,the expression of HSP70 at 1 h,24 h,36 h after trauma was higher than group of trauma especially at 1 h（P〈0.05）.Conclusion Ulinastatin can reduce the risk of renal injury in early times after trauma especially at 1 h.The protective effects of ulinastatin may be associated with the restrict the expression of IL-6,up-regulation of HSP70 expression,and change the proportion between IL-6 and HSP70 expression.

关 键 词：肾脏 创伤 乌司他丁 IL-6 HSP70 组织芯片 

分 类 号：R692[医药卫生—泌尿科学]