上皮性卵巢癌AIB1蛋白表达水平及其与凋亡相关性的初步研究  

Expression of AIB1 Protein in Epithelial Ovarian Cancer Cells and Its Impact on Apoptosis

作　　者：韩肖燕[1] 陈悦[1] 侯敏敏[1] 张健[1] 杨开选[2] 陈莹莹[1] 郄明蓉[1] 

机构地区：[1]四川大学华西第二医院妇产科,成都610041 [2]四川大学华西第二医院病理科

出　　处：《四川大学学报（医学版）》2008年第4期619-622,634,共5页Journal of Sichuan University(Medical Sciences)

基　　金：高等学校博士学科点专项科研基金(20060610080)资助

摘　　要：目的观察AIB1蛋白在上皮性卵巢癌中的表达,并探讨其与凋亡调节蛋白P53、Bcl-2表达之间的关系。方法应用免疫组化SP法检测24例正常卵巢、24例卵巢良性肿瘤、18例卵巢交界性肿瘤及69例上皮性卵巢癌中AIB1、P53、Bcl-2蛋白的表达,同时测定卵巢癌中雌激素受体(ER)、孕激素受体(PR)状态,分析AIB1的表达与P53蛋白、Bcl-2蛋白、ER、PR的表达间有无相关性。结果1AIB1在正常卵巢、卵巢良性肿瘤、卵巢交界性肿瘤、上皮性卵巢癌中阳性表达率分别为8.33%、25.00%、38.89%、65.22%,卵巢癌中AIB1蛋白的阳性表达率高于其他卵巢组织(P<0.05)。AIB1蛋白在卵巢癌中过表达的发生率为36.23%,低分化、临床晚期、淋巴结受累的病例较之中、高分化、临床早期、无淋巴结转移的病例AIB1蛋白的过表达发生率升高(P<0.05)。2相关性分析显示AIB1蛋白与P53、Bcl-2蛋白表达呈正相关(r=0.342,P=0.004;r=0.311,P=0.009),与ER、PR的表达无相关性。结论上皮性卵巢癌中,AIB1蛋白的表达率明显升高,与卵巢癌的恶性生物学行为关系密切。AIB1蛋白可能通过非激素依赖途径影响了凋亡调节蛋白如P53、Bcl-2的表达,从而参与了上皮性卵巢癌的发生发展过程。Objective To investigate the expression of AIB1（amplified in breast cancer 1）protein in epithelial ovarian cancer cells and to analyze the relationship between AIB1 protein and the apoptosis modulated proteins：P53 and Bcl-2.Methods The expressions of AIB1,P53 and Bcl-2 proteins in 24 normal ovaries,24 benign ovarian tumors,18 borderline ovarian tumors and 69 epithelial ovarian cancers were examined with immunohistochemical method SP.The expressions of estrogen receptor（ER）and progesterone receptor（PR）were also examined to reveal their relation with AIB1 expression.Results ① The AIB1 protein expressed in 65.22% ovarian cancers,greater than in normal ovaries（8.33%）,benign ovarian tumors（25.00%）and borderline ovarian tumors（38.89%）.The overexpression of AIB1 protein occurred in 36.23% ovarian cancers.Ovarian cancers poorly-differentiated,at a late clinical stage and with lymph node involvement had higher AIB1 overexpression than those well-differentiated and moderately-differentiated,and those at an early clinical stage and without lymph node involvement.② The expression of AIB1 was positively correlated with the expression of P53（r=0.342,P=0.004）and Bcl-2 proteins（r=0.311,P=0.009）in the ovarian cancers.There was no correlations between the AIB1 expression and the ER and PR.Conclusion AIB1 protein expression increases in ovarian cancers,which is associated with the higher malignant biological behavior.The overexpression of AIB1 may be involved in the carcinogenesis and the development of epithelial ovarian cancers through a hormone independent pathway.Our findings suggest a possible correlation between AIB1 and apoptosis modulated proteins P53 and Bcl-2.

关 键 词：卵巢肿瘤 AIB1蛋白 P53蛋白 BCL-2蛋白 免疫组化 

分 类 号：R737.31[医药卫生—肿瘤]