口蹄疫病毒P12A^＊3C基因转化饲草玉米高效表达载体的构建  

Construction of highly efficient expression vector with food-and-mouth disease virus P12A~*3C gene for transformation of the gene into maize

作　　者：张素芝[1] 潘丽[1] 荣廷昭[2] 张永光[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点实验室,甘肃兰州730046 [2]四川农业大学玉米研究所作物基因资源与遗传改良教育部重点实验室,四川雅安625014

出　　处：《中国兽医科学》2008年第7期549-554,共6页Chinese Veterinary Science

基　　金：国家“十一五”高技术研究发展计划(863)项目(2006AA10A204);四川省公益性研究计划项目(2008NG0013);四川农业大学博士后基金项目;四川农业大学青年科技创新基金项目;四川省应用基础研究计划项目(2008JY0096);四川省教育厅资助科研项目(07ZB065)

摘　　要：为了研究饲草玉米可饲口蹄疫疫苗,将O型口蹄疫病毒的VP1和2A基因序列按照玉米的偏爱性密码子优化后,再与P1的其他基因及3C基因连接。同时,通过使用高效的Ubiquitin启动子以及在P1的起始密码子处添加Kozak序列和在3C基因3′末端添加内质网引导肽序列等措施提高免疫原基因的表达。结果显示,高效植物表达载体pC1300P12A*3C构建正确,可以用于后续的饲草玉米可饲口蹄疫疫苗的研究。To study the transgenic oral vaccine against food-and-mouth disease,the sequences of VP1 gene and 2A gene of food-and-mouth disease virus（FMDV） type O were optimized according to the codon bias of maize and jointed to the other gene of P1 and 3C genes. A series of measures,such as using highly efficient promoter of Ubiqultin ,adding Kozak sequence to the initial codon of P1 gene and adding SEKD EL endoplasmic reticulum signal peptide sequence into the 3′ gene terminal of 3C gene were taken to improve the expression level of the immunogen. The results showed that the highly efficient expression vector pC1300P12A^＊3C was constructed correctly and it was suitable for the subsequent exploration of oral vaccine against FMDV by transformation of maize.

关 键 词：口蹄疫病毒 饲草玉米 可饲疫苗 高效表达载体 

分 类 号：S852.659.6[农业科学—基础兽医学]