羊O／P液中AsiaⅠ型口蹄疫病毒VP1基因3′端序列的分析  

Analysis of VP1 gene 3′-terminal sequence of foot-and-mouth disease virus type AsiaⅠ derived from ovine O/P fluid

作　　者：王建东[1] 卢曾军[1] 包慧芳[1] 曹轶梅[1] 郭建宏[1] 刘湘涛[1] 何生虎[2] 杨春生[2] 刘在新[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点实验室国家口蹄疫参考实验室,甘肃兰州730046 [2]宁夏大学农学院,宁夏银川750001

出　　处：《中国兽医科学》2008年第7期559-562,共4页Chinese Veterinary Science

基　　金：国家“十一五”科技支撑计划项目(2006BAD06A10);国家重点基础研究发展计划(973)项目(2005CB523201)

摘　　要：从宁夏中卫AsiaⅠ型口蹄疫疫区的无临床症状羊采集的食道咽部分泌液（OPF）中扩增得到了4个口蹄疫病毒（FMDV）VP1基因3′-端483bp（VP483）片段。核苷酸和氨基酸序列比较结果表明，从4份OPF中扩增出的VP1片段，在细胞受体结合位点处的关键氨基酸与目前公布的FMDVAsiaⅠ型流行毒相比均发生了改变，由RGD变成RDD。4个扩增片段的核苷酸序列与AsiaⅠ／js／WX／05株相比，同源性为96．2％～98、3％，表明与AsiaI／JS／WX／05株高度同源。蛋白结构模拟结果显示，这种改变会导致G—H环柔性减弱，这可能是AsiaⅠ／JS／WX／05FMDV适应羊体，造成持续感染后发生的一种变异。Four fragments representing foot-and-mouth disease virus （FMDV） VP1 gene 3′-terminal 483 bp fragments（VP483） were amplified from oesophageal-pharyngeal fluids（OPF） of sheep in a FMD Asia Ⅰ epidemic area in Zhongwei of Ningxia Province. The nucleotide sequences and amino acid sequences were compared with those of reference sequences. The key amino acids existing in cell receptor binding sites were changed from RGD to RDD compared to Asia Ⅰ/JS/WX/05 lineage of FMDV, which was a prevalent strain of FMDV Asia Ⅰ in China. The four fragments shared 96.2% to 98.3% identities with that of Asia Ⅰ/ JS/WX/05 strain. In order to analyse the effect of such change on biological characters of Asia Ⅰ/JS/WX/ 05 strain, the RGD tripeptide in Asia Ⅰ/JS/WX/05 were replaced with RDD ior modeling its three- dimensional structure. The result showed that the flexibility of G-H loop went down after such change. This study demonstrated variation of FMDV Asia Ⅰ/JS/WX/05 strain after persistent infection in sheep.

关 键 词：AsiaⅠ型口蹄疫病毒 羊O/P液 VPl基因 序列分析 

分 类 号：S852.659.6[农业科学—基础兽医学]