高压氧及常压氧干预大鼠超长随意皮瓣细胞成纤维细胞生长因子1及转化生长因子β_1的表达  

Effects of hyperbaric oxygen and normobaric oxygen on levels of fibroblast growth factor-1 and transforming growth factor beta-1 in rat models of ultra-long random flap

作　　者：吴琍[1] 任纪祯[1] 苗嫄昕[1] 

机构地区：[1]青岛大学医学院附属医院,山东省青岛市266003

出　　处：《中国组织工程研究与临床康复》2008年第31期6077-6081,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:常规皮瓣设计常遵循长∶宽≤2∶1的原则,在某些局部血运丰富的区域,可达3∶1,超出这个范围,皮瓣的远端易发生坏死。如何能实现超长随意皮肤皮瓣的一次转移成功,是组织移植希望解决的问题。目的:将超长随意皮瓣设计为6∶1,观察高压氧、常压氧对大鼠随意皮瓣细胞成纤维细胞生长因子1及转化生长因子β1水平的影响。设计、时间及地点:随机分组,组织细胞移植的对比观察实验,于2006-03/09在青岛大学医学院附属医院动物试验中心完成。材料:4月龄Wistar雄性大鼠60只随机分为3组。方法:每组于背部中线形成一蒂在尾端的超长随意皮瓣1cm×6cm大小,对照组不给予干预,常压氧组术后当天给予常压氧2次,每次1h,连续5d,高压氧组术后当天给予高压氧2次,每次1h,持续5d。主要观察指标:术后7d计算各组皮瓣成活率,并监测皮瓣组织中成纤维细胞生长因子1及转化生长因子β1的表达量。结果:高压氧组皮瓣成活率较常压氧组与对照组明显提高,免疫组织化学检测高压氧组中成纤维细胞生长因子1的表达均较其余两组为高,提示皮瓣成活率的提高可能与成纤维细胞生长因子的高表达有关,转化生长因子β1的表达3组比较无明显差异。结论:高压氧干预组皮瓣成活率高,皮瓣细胞中成纤维细胞生长因子表达较常压氧组明显提高,而转化生长因子β1表达3组比较无差异。提示转化生长因子β1在皮瓣存活的中的作用不明显。BACKGROUND： Ratio of surgical flap in usual is less than or equal to 2：1, and it can be 3：1 in some particular place where has abundant blood supply, the exceeded would necrose. How to make successful ultra-long random flap transportation deserves a good observation. OBJECTIVE： To study the effects of hyperbaric oxygen and normobaric oxygen on levels of fibroblast growth factor （FGF）- 1 and transforming growth factor （TGF） β 1 in a 6：1 rat random-pattern flap model. DESIGN, TIME AND SETTING： The cell transplantation experiment was conducted at the Animal Experimental Center of Affiliated Hospital of Medical College, Qingdao University from March to September 2006 by randomized grouping method. MATERIALS： Sixty male Wistar rats aged 4 months were randomized into 3 groups. METHODS： A 1 cm×6 cm free flap was elevated in the central part of the rachis. Rats in the control group were intact. Rats in the normobaric oxygen group received normobaric oxygen twice, once for 1 hour, totally successively 5 days. Rats in the hyperbaric oxygen group received hyperbaric oxygen twice, once for 1 hour, totally successively 5 days. MAIN OUTCOME MEASURES： Survival rate of the flap in each group was calculated and levels of FGF-1 and TGF β 1 were measured 7 days after surgery. RESULTS： The survival rate was significantly higher in the hyperbaric oxygen group than in the normobaric oxygen group and control group. Immunohistochemistry showed that FGF-1 level was much higher in the hyperbaric oxygen group than in the normobaric oxygen group and control group. This indicated that the elevated survival rate of the flap was associated with high expression of FGF-1. There was no significant difference in TGF β 1 expression in the three groups. CONCLUSION： In the hyperbaric oxygen group, survival rate of the flap is high and FGF-1 levels are elevated compared with the normobaric oxygen. No significant difference in TGF β 1 expression is detected in the three groups. This indicated that TGF β

关 键 词：高压氧 常压氧 大鼠 随意皮瓣 成纤维细胞生长因子1 转化生长因子Β1 

分 类 号：R459.6[医药卫生—治疗学]