羟基磷灰石超微粉对肿瘤细胞系 Walker 256 的 DNA 损伤  

The Role of Hydroxyapatite Ultrafinepowder in DNA Damage of the Tumor Cell Line Walker 256

作　　者：张建平[1] 夏清华[1] 田元[1] 陈道达[1] 

机构地区：[1]同济医科大学附属协和医院

出　　处：《实用癌症杂志》1997年第4期245-247,共3页The Practical Journal of Cancer

摘　　要：作者采用单细胞凝胶电泳技术检测不同剂量羟基磷灰石（ＨＡ）超微粉对肿瘤细胞系Ｗａｌｋｅｒ２５６的ＤＮＡ损伤，实验显示ＤＮＡ损伤随ＨＡ剂量增加而加强。当ＨＡ剂量＞２００μｇ／ｍｌ时，ＤＮＡ链缺口较对照组显著增加（Ｐ＜０．０５）。随剂量进一步加大，伴随细胞毒作用增强。表明ＨＡ对肿瘤细胞系Ｗａｌｋｅｒ２５６同时具有基因毒和细胞毒作用，显示其抗肿瘤价值。The authors measured DNA damage of tumor cell line Walker 256 exposed to Hydroxyapatite (HA) ultrafinepowder using comet assay. The experiment demonstrated a significant increase of DNA strand breaks when HA dosage was higher than 200 μg/ml (P<0.05). As dosage of HA further increased, the cytotoxicity increased accordingly, indicating the antitumor role of HA.

关 键 词：羟基磷灰石超微粉 单细胞凝胶电泳 DNA损伤 

分 类 号：R979.1[医药卫生—药品]