一株纤维素降解新菌种发酵玉米秸秆的生物产氢特性研究  

SIMULTANEOUS BIO-HYDROGEN PRODUCTION AND PRETREATED CORN STALK DEGRADATION BY A NEW CELLULOSE-DEGRADATION BACTERIUM

作　　者：包红旭[1] 任南琪[1] 王爱杰[1] 

机构地区：[1]哈尔滨工业大学市政环境工程学院,哈尔滨150090

出　　处：《太阳能学报》2008年第7期775-780,共6页Acta Energiae Solaris Sinica

基　　金：国家重点基础研究发展规划(973)项目(G2000026402);国家自然科学基金(30470054)

摘　　要：试验从连续流发酵产氢反应器(ZL92114474.1)中分离筛选出一株高效纤维素降解产氢细菌Clostridium sp.X9。X9利用微晶纤维素(MC)作为发酵产氢底物,得到最大单位体积产氢量(Y_(H_2))、比产氢率(Y_(H_2)/s)和纤维索降解率分别为780 mL H_2/L-culture、5.1 mmol H_2/g-cellulose和69.6%。采用酸、碱、氨水和酸化汽爆方式预处理玉米秸秆,结果表明,酸化汽爆方式可以获得最佳的预处理效果。X9利用酸化汽爆玉米秸秆(cSES)发酵产氢的Y_(H_2)、Y_(H_2)/s和纤维素降解率分别达到730 mL H_2/L-culture、4.3mmol H_2/g-cellulose和64%。这说明新菌种X9在利用玉米秸秆类生物质纤维素发酵产氢方面具有很好的应用潜力。In this study, Cellulolyticum sp. X9, anaerobic high efficiency cellulose degrading and hydrogen producing bacterium, was isolated from a continuous-flow H2 producing reactor（ZL92114474.1 ）. Experimental results showed that strain X9 has high hydrogen yield （ YH2 ） of 780 ml H2/L-culture, specific H2 production rate （YH2 /s） of 5. 1 mmol H2/ g-cellulose and cellulose degradation degree of 69.6% feeding with microcrystalline cellulose （MC）. Then, strain X9 was adopted to investigate H2 production capabilities directly from corn stalks fermentation following with four different pretreatment methods, Of which, steam explosion pretreatment showed the highest H2 yield （ YH2 ） and corn stalk degradation degree. This indicated that Cellulolyticum sp. X9 has great potential for fermentative H2 production from lignocellulose biomass like corn stalks after effective pretreatment.

关 键 词：生物制氢 产氢细菌 玉米秸秆 预处理 纤维素降解 

分 类 号：S216[农业科学—农业机械化工程]