产氢菌的投加方式对强化发酵菌群产氢的影响  

EFFECTS OF ADDITION METHOD OF HYDROGEN PRODUCING BACTERIA ON THE BIOAUGMENTATION OF FERMENTATIVE MICORFLORA

作　　者：秦智[1,2] 任南琪[2] 李建政[2] 

机构地区：[1]哈尔滨理工大学化学与环境工程学院,哈尔滨150040 [2]哈尔滨工业大学市政环境工程学院,哈尔滨150090

出　　处：《太阳能学报》2008年第7期883-887,共5页Acta Energiae Solaris Sinica

基　　金：国家高技术研究发展计划(863)项目(2006AA05Z109);国家杰出青年基金项目(50125823);黑龙江省自然科学基金项目(E2004-08);黑龙江省教育厅科研项目(10541053);哈尔滨市青年基金项目(2004AFQXJ041)

摘　　要：通过间歇培养研究了产氢菌Ethanoligenens sp B49的投加方式对生物制氢反应器的混合发酵菌群生物强化作用的影响。结果表明,产氢菌的投加方式对发酵菌群的产氢能力有显著影响。产氢菌发酵液的直接投加使发酵菌群的产氢能力下降,并引起培养液中发酵产物乙醇和乙酸浓度的显著增加。分析认为,产氢菌发酵液对发酵菌群的末端产物抑制和低pH值抑制作用是导致产氢作用受到抑制的主要原因。离心后单独投加产氢菌菌体可提高发酵菌群的产氢能力,起到强化产氢的作用。投加10.8%的产氢菌强化发酵菌群时,培养45h的累计产氢量为155.0 mL,比强化前发酵菌群培养的产氢量提高了21.5%。因此在利用产氢菌生物强化发酵菌群的研究中,应采用离心分离后单独投加产氢菌菌体的方式进行生物强化。The addition method of hydrogen producing bacteria（HPB） B49 for bioaugmentation of fermentative micorflora in batch culture was investigated. It was demonstrated that addition method had great effects on the hydrogen producing capacity of fermentative microflora. Direct addition of fermented broth with HPB inhibited hydrogen production, and increased the concentration of ethanol and acetic acid in fermentative products significantly. The inhabitation of fermentative products and low pH value caused by fermented broth were the main reasons for the hydrogen producing inhibition of fermentative microflora. Addition of HPB after centrifuge enhanced hydrogen production of microflora. Addition of 10.8% HPB into fermentative microflora, hydrogen production yield reached 155.0mL after culture of 45 hours, improved 21. 5 % than before bioaugmentation. Therefore, the addition method of centrifuging HPB should be adopted for bioaugmentation.

关 键 词：生物制氢 生物强化 产氢菌 发酵菌群 投加方式 

分 类 号：X382[环境科学与工程—环境工程]