小球藻psbA基因的克隆与序列分析  

Cloning and Analyses of psbA Gene from Unicellular Green Alga Chlorella

作　　者：吴晓微[1] 孙雪[1] 陆开形[1] 汪一冰[1] 张晓龙[1] 

机构地区：[1]宁波大学生命科学与生物工程学院,教育部应用海洋生物工程重点实验室,浙江宁波315211

出　　处：《水产科学》2008年第7期360-362,共3页Fisheries Science

基　　金：浙江省教育厅资助项目(20040899);宁波市自然科学基金资助项目(2006A610084);宁波大学科研基金资助项目(XK200534)

摘　　要：psbA基因编码光合系统Ⅱ反应中心的D1蛋白，是叶绿体基因组中一个重要的光调控基因。对小球藻属4个种的6株小球藻psbA基因进行了PCR扩增和测序，并结合GenBank上已有的2条小球藻psbA基因序列，使用MEGA3.1软件对psbA序列进行了遗传距离值的计算和系统发育树的构建。结果显示8株小球藻psbA基因的遗传距离值为0．012～0．167。除了原始小球藻F-2和蛋白核小球藻820分别与蛋白核小球藻F-5、F-9及与普通小球藻Cvq关系极近之外，其他6株小球藻基本上按照种的划分进行聚类，该结果与用小球藻的ITS和rbcL序列分析的结果一致。The psbA gene coding for D1 protein in photosynthetic Ⅱ reaction centre is one of the important lightregulated genes in the chloroplast genome. The psbA genes from 6 strains in genus Chlorella were amplified by PCR and sequenced. Combined with the other two psbA sequences in the GenBank database, the distances in 8 psbA sequences were calculated and phylogenetic tree was constructed using MEGA3.1 software. The results showed that the genetic distances in the 8 Chlorella strains ranged from 0.012 to 0.167. The 8 strains of Chlorella were basically clustered according to their species relationships except for C. protothecoides F- 2 and C. pyrenoidosa 820, which showed very close relationship with C. pyrenoidosa F- 5 and F-9 and with C. vulgaris Cvq, respectively. This results were consistent with those obtained from the ITS or rbcL sequences of Chlorella.

关 键 词：小球藻 PSBA 遗传距离 系统树 

分 类 号：Q781[生物学—分子生物学]