隐孔菌多糖对脂多糖诱导人肺上皮细胞生成单核细胞趋化蛋白-1的作用  

Effect of Cryptoporus polysaccharide on lipopolysaccharide-induced production of monocyte chemoattractant protein-1 in human alveolar epithelial cells

作　　者：朱剑萍[1] 章辉[1] 谢强敏[2] 

机构地区：[1]浙江大学医学院附属邵逸夫医院,浙江杭州310016 [2]浙江大学国家食品药品监督管理局浙江呼吸药物研究实验室,浙江杭州310058

出　　处：《浙江大学学报（医学版）》2008年第4期333-339,共7页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省浙江省科技厅重点科研项目(2005C21072);国家自然科学基金资助项目(30772581)

摘　　要：目的:研究隐孔菌多糖(CP)对脂多糖(LPS)诱导人肺上皮细胞株A549生成单核细胞趋化蛋白-1(MCP-1)的影响。方法:在有或无CP条件下用LPS诱导A549细胞株,分别采用ELISA和RT-PCR法,测定MCP-1蛋白浓度和MCP-1 mRNA的表达。结果:LPS 1000μg/L诱导A549细胞24 h明显增加MCP-1的生成。CP 100μg/L或地塞米松1μmol/L对A549细胞株的生长和活力无明显影响。CP 100μg/L或地塞米松1μmol/L能明显抑制LPS诱导A549细胞株的MCP-1蛋白含量和mRNA的表达。结论:结果证明,CP能调节MCP-1的生成,可能是其抗肺部炎症的作用机制之一。Objective： To investigate the effect of cryptoporus polysaccharide （CP） on lipopolysaccharide （LPS）-induced production of monocyte chemoattractant protein-1 （MCP 1）in human lung epithelial A549 cells. Methods： A549 cells were stimulated with LPS in the presence or absence of CP. The protein concentration and mRNA expression of MCP-1 were determined by enzyme-linked immunosobent assay （ELISA） and semi-quantitative RT-PCR, respectively. Results： The protein concentration of MCP-1 was significantly increased by LPS 1000 μg/L at 24 h. There were no effects on the growth and viability of A549 cells in the presence of CP 100 μg/ L or dexamethasone 1 μmol/L. However, CP 100 μg/L or dexamethasone 1μmol/L significantly inhibited the protein concentration and mRNA expression of MCP-1 induced by LPS. Conclusion： CP can regulate MCP-1 production, which may be associated with its effects on lung inflammation.

关 键 词：多糖类 细菌/药理学 肺炎/病理学 隐孔菌多糖 肺上皮细胞 单核细胞趋化蛋白-1 

分 类 号：R563[医药卫生—呼吸系统]