留兰香油对大鼠慢性阻塞性肺疾病模型肺组织炎症氧化改变和Nrf2蛋白表达的影响  

Effect of Spearmint oil on inflammation,oxidative alteration and Nrf2 expression in lung tissue of COPD rats

作　　者：赵春贞[1] 王砚[1] 唐法娣[1] 赵小京[1] 徐巧萍[1] 夏锦芳[1] 朱有法[1] 

机构地区：[1]浙江大学医学院国家食品药品监督管理局浙江呼吸药物研究实验室,浙江杭州310058

出　　处：《浙江大学学报（医学版）》2008年第4期357-363,共7页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省科技计划资助项目(2008c23066)

摘　　要：目的：观察肺炎克雷伯杆菌和脂多糖所致大鼠慢性阻塞性肺疾病（COPD）模型肺组织炎症氧化改变，以及Nrf2蛋白表达及留兰香油的影响。方法：用经气管滴入脂多糖和肺炎克雷伯杆菌反复感染法12周建立大鼠COPD模型后，留兰香油灌胃治疗3周。观察大鼠COPD模型病理学变化、支气管肺泡灌洗液（BALF）中白细胞数变化、肺组织匀浆MDA变化、免疫组化检测Nrf2蛋白表达特点，以及留兰香油对其的影响。结果：留兰香油100mg·kg^-1能明显降低BALF中白细胞数，减轻细支气管炎和肺间质炎及炎症细胞浸润；30～300mg·kg^-1能减少肺泡破坏，使细支气管壁变薄以及抑制杯状细胞增生；留兰香油能明显降低肺组织MDA含量，抗氧化效果明显，而且30mg·kg^-1可降低肺组织Nrf2蛋白表达。结论：留兰香油对肺炎克雷伯杆菌和脂多糖引起的大鼠COPD模型的肺部炎症、肺气肿、杯状细胞增生和氧化改变有改善作用，并对肺炎克雷伯杆菌和LPS引起的肺组织Nrf2蛋白表达增强有降低作用。Objective： To investigate the effect of Spearmint oil on inflammation, oxidative alteration and Nrf2 expression in rats with chronic obstructive pulmonary disease （COPD）. Methods： COPD model was induced by intratracheal instillation of Klebsiella pneumonia and lipopolysaccharide （LPS） for 12 weeks in rats, and COPD rats were treated with Spearmint oil for 3 weeks. After COPD was induced, the pathological changes, changes in leucocyte number in blood and bronchoalveolar lavage fluid （BALF）, MDA in lung homogenate and Nrf2 expression were observed. The effects of Spearmint oil on these changes were determined. Results：Spearmint oil 100 mg · kg ^-1 significantly reduced leucocyte numbers in BALF, and attenuated bronchiolitis, pulmonary interstitial inflammation and inflammation cell infiltration. Spearmint oil 30-300 mg · kg^-1 decreased the destruction of pulmonary alveolus and the thickness of bronchioles walls, and inhibited goblet cell proliferation. Spearmint oil significantly reduced MDA in lung homogenate, and decreased the expression of Nrf2 protein in lung tissues. Conclusion： Spearmint oil has protective effect on lung injury in COPD rats, since it improves pulmonary inflammation,oxidative alteration, and enhances Nrf2 protein expression.

关 键 词：油类 挥发/药理学 肺气肿/病理学 肺疾病 慢性阻塞性/药物疗法 克雷伯菌 肺炎 留兰香油 脂多糖 Nrf2蛋白 

分 类 号：R563.9[医药卫生—呼吸系统] R96[医药卫生—内科学]