核桃苯丙氨酸解氨酶的基因克隆与序列分析  

Molecular Cloning and Sequence Analysis of A Phenylalanine Ammonia-lyase Gene from Juglans regia

作　　者：王燕[1] 张风霞[1] 朱俊[1] 李琳玲[2] 许锋[1] 程水源[2] 

机构地区：[1]长江大学园艺园林学院,湖北荆州434025 [2]黄冈师范学院生命科学与工程学院,湖北黄州438000

出　　处：《湖北农业科学》2008年第6期622-626,共5页Hubei Agricultural Sciences

基　　金：教育部新世纪优秀人才计划项目(NCET-04-0746);湖北省自然科学基金项目(2002AB094);湖北省自然科学青年杰出人才基金项目(2003AB014);湖北省教育厅重大科技项目(Z200627002)

摘　　要：利用1对兼并引物,从核桃(Juglans regia)中克隆得到苯丙氨酸解氨酶(PAL)基因cDNA片段,并命名为JrPAL,GenBank登录号为AY747676。JrPAL长866bp,编码289个氨基酸。通过核苷酸和蛋白质序列多重比较,发现JrPAL与其他植物的PAL基因高度同源。JrPAL编码的蛋白质序列包含与水稻、玉米PAL蛋白质相同的脱氨基位点和催化活性位点。PAL系统进化树表明,JrPAL与乔木类植物的PAL基因聚类关系最近。克隆JrPAL,可为利用基因工程技术调控核桃黄酮化合物的代谢提供基因资源。A phenylalanine ammonia-lyase （PAL） gene cDNA was cloned from Juglans regia using a pair of degenerated primers. The PAL cDNA fragment of J. regia （designated as JrPAL） was 866 bp length and it encoded a 289 amino acid protein. JrPAL was found to have extensive homology with those of other plant PAL genes via multiple alignments. The deamination sites and catalytic active sites in PAL protein of Oryza sativa and Zea mays were also found in JrPAL. Phylogenetic tree analysis revealed that JrPAL had closer relationship with PALs from arbor plants than from other plants. JrPAL was a useful tool to study the regulation of flavonoid metabolism in J. regia.

关 键 词：核桃 苯丙氨酸解氨酶基因 基因克隆 序列分析 

分 类 号：S664.1[农业科学—果树学] Q785[农业科学—园艺学]