3种脱除南瓜水溶性多糖蛋白方法的比较  

Comparison of Three Methods for Removal of Protein from Pumpkin Polysaccharide

作　　者：李俊丽[1] 王运强[1] 向长萍[1] 

机构地区：[1]华中农业大学园艺林学学院/国家蔬菜改良中心华中分中心

出　　处：《湖北农业科学》2008年第6期717-719,共3页Hubei Agricultural Sciences

基　　金：湖北省三峡移民局资助项目

摘　　要：探讨了三氯乙酸法、重金属离子沉淀法和Sevag法3种方法对南瓜多糖提取物进行脱除蛋白处理的效果。结果表明,综合考虑多糖损失率、脱除蛋白率和活性等因素,Sevag法优于三氯乙酸法和重金属离子沉淀法,是适用于南瓜水溶性多糖脱除蛋白的有效方法。In this study,three methods （trichloroacetic acid method, Cu^2＋ method and Sevag method） were used to remove protein from pumpkin polysaccharide. The results showed that Sevag method was better than other two methods to remove protein from pumpkin polysaccharide, which was more effective in the removal percentage of protein, the loss of polysaccharide and physiological activity of polysaccharide.

关 键 词：南瓜 多糖 游离蛋白 

分 类 号：Q539[生物学—生物化学] Q591.2