Hematological Changes in White Spot Syndrome Virus-Infected Shrimp,Fenneropenaeus chinensis(Osbeck)  

作　　者：FENG Shouming ZHAN Wenbin XING Jing LI Jun YANG Kai WANG Jing 

机构地区：[1]Laboratory of Pathology and Immunology of Aquatic Animals, LMMEC, Ocean University of China, Qingdao 266003, P. R China [2]Tianjin Fishery Institute, Tianjin 300221, P. R. China

出　　处：《Journal of Ocean University of China》2008年第3期287-293,共7页中国海洋大学学报（英文版）

摘　　要：The pathological changes of hemocytes in the haemolymph and hepatopancreas were examined in experimentally and naturally WSSV(white spot syndrome virus) infected Fenneropenaeus chinensis. The results showed that the pathological manifesta-tions of hemocytes were similar among moribund shrimps infected via injection,feeding and by nature. Firstly,the total hemocyte counts(THCs) in WSSV-infected shrimp were significantly lower than those in healthy shrimp. Secondly,necrotic,broken and dis-integrated cells were often observed,and a typical hematolysis was present in the haemolymph smear of WSSV-infected shrimp. Thirdly,necrosis and typical apoptosis of hemocytes were detected with TEM in the peripheral haemolymph of WSSV-infected shrimp. Hyalinocytes and semi-granulocytes with masses of WSSVs in their nuclei often appeared,whereas no granular hemocytes with WSSV were found in the hepatopancreas of moribund infected shrimps. All our results supported that hemocytes were the main target cells of WSSV,and hyalinocytes and semigranular hemocytes seemed to be more favorable for WSSV infection in F. chinensis.The pathological changes of hemocytes in the haemolymph and hepatopancreas were examined in experimentally and naturally WSSV （white spot syndrome virus） infected Fenneropenaeus chinensis. The results showed that the pathological manifestations of hemocytes were similar among moribund shrimps infected via injection, feeding and by nature. Firstly, the total hemocyte counts （THCs） in WSSV-infected shrimp were significantly lower than those in healthy shrimp. Secondly, necrotic, broken and disintegrated cells were often observed, and a typical hematolysis was present in the haemolymph smear of WSSV-infected shrimp. Thirdly, necrosis and typical apoptosis of hemocytes were detected with TEM in the peripheral haemolymph of WSSV-infected shrimp. Hyalinocytes and semi-granulocytes with masses of WSSVs in their nuclei often appeared, whereas no granular hemocytes with WSSV were found in the hepatopancreas of moribund infected shrimps. All our results supported that hemocytes were the main target cells of WSSV, and hyalinocytes and semigranular hemocytes seemed to be more favorable for WSSV infection in F. chinensis.

关 键 词：HEMOCYTE pathological changes WSSV Fenneropenaeus chinensis （Osbeck） 

分 类 号：Q959.223[生物学—动物学]