Identification of a Putative Tetrasporophyte-Specific Gene in Gracilaria lemaneiformis(Gracilariales,Rhodophyte)  

Identification of a Putative Tetrasporophyte-Specific Gene in Gracilaria lemaneif ormis （Gracflariales, Rhodophyte）

作　　者：REN Xueying ZHANG Xuecheng 

机构地区：[1]College of Marine Life Sciences, Ocean University of China, Qingdao 266003, P. R. China

出　　处：《Journal of Ocean University of China》2008年第3期299-303,共5页中国海洋大学学报（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(Nos.30170736,30671603 and 40606034).

摘　　要：A putative tetrasporophyte-specific gene,designated as SSH466(GenBank accession No. DQ019223) ,was one of the genes identified in this work using suppression subtractive hybridization(SSH) method in Gracilaria lemaneiformis. The full length of the gene was obtained using SMART RACE strategy. Sequence analysis revealed that the gene had 1 019 nucleotides,including an open reading frame of 498 nucleotides encoding 166 amino acid residues,158 nucleotides of 5' untranslated region and 363 nucleotides of 3' non-coding region. Protein motif and secondary structure prediction showed that there existed a transmembrane domain with a unique β-sheet. Thus,SSH466 protein might be a cross-membrane protein. Sequence homology search in the public GenBank databases did not reveal any significant match with SSH466. Virtual Northern blot analysis confirmed that it was a tetrasporophyte-specific gene.A putative tetrasporophyte-specific gene, designated as SSH466 （GenBank accession No. DQ019223）, was one of the genes identified in this work using suppression subtractive hybridization （SSH） method in Gracilaria lemaneiformis. The full length of the gene was obtained using SMART RACE strategy. Sequence analysis revealed that the gene had 1 019 nucleotides, including an open reading frame of 498 nucleotides encoding 166 amino acid residues, 158 nucleotides of 5＇ untranslated region and 363 nucleotides of 3＇ non-coding region. Protein motif and secondary structure prediction showed that there existed a transmembrane domain with a unique β-sheet. Thus, SSH466 protein might be a cross-membrane protein. Sequence homology search in the public GenBank databases did not reveal any significant match with SSH466. Virtual Northern blot analysis confirmed that it was a tetrasporophyte-specific gene.

关 键 词：suppression subtractive hybridization （SSH） TETRASPOROPHYTE female gametophyte Virtual Northern blot 

分 类 号：Q949.2[生物学—植物学]