针刺对快速老化骨质疏松小鼠P6骨代谢因子OPG、BMP-2的影响  

Effects of acupuncture on expression of OPG and BMP-2 in SAMP6 mouse with osteoporosis

作　　者：张雪竹[1] 彭应梅[1] 于建春[1] 刘存志[1] 韩景献[1] 

机构地区：[1]天津中医药大学第一附属医院针灸研究所,天津300193

出　　处：《中华中医药杂志》2008年第8期672-675,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金重点项目(No.30630074);天津市应用基础及前沿技术研究计划重点项目(No.07JCZDJC08800);教育部高等学校博士学科点专项科研基金(No.20060063006);天津市卫生局中医;中西医结合科研专项资金课题(No.2005034;No.07059);天津市高等学校科技发展基金计划项目(No.20060306)

摘　　要：目的:探讨针刺治疗骨质疏松的机制。方法:以快速老化骨质疏松小鼠P6(SAMP6)及正常同源抗快速老化小鼠R1(SAMR1)为模型,采用Western Blot等方法,观察SAMP6对照组、SAMP6针刺组、SAMP6非穴位刺激组和SAMR1对照组股骨护骨素(OPG)、骨形态发生蛋白-2(BMP-2)蛋白表达的变化。结果:SAMP6对照组股骨OPG、BMP-2表达显著下调(P<0.05);针刺后两者表达上调并趋于正常(P<0.05)。结论:SAMP6小鼠发生骨质疏松与OPG、BMP-2等骨代谢因子表达异常有关,针刺可通过促进成骨细胞形成并分泌骨代谢因子来刺激骨形成,降低骨转换率,达到改善骨质疏松的作用。Objective： To explore the mechanisms of acupuncture in improving osteoporosis. Methods： In this study, the expression of OPG and BMP-2 proteins of femur in SAMP6 control group, SAMP6 acupuncture group, SAMP6 sham-acupuncture group and SAMR1 control group were determined by Western Blot technique. Results： Compared with SAMR1 control group, the expression of OPG and BMP-2 proteins of the femur in SAMP6 control mice decreased obviously. Acupuncture could stimulate the expression of OPG and BMP-2 and no differences were detected between the SAMR1 control group and SAMP6 acupuncture group. Conclusion： Osteoporosis observed in SAMP6 was correlated with low expression of OPG and BMP-2. After acupuncture treatment, both of them showed increased expression, it suggested that the curative effects of acupuncture on osteoporosis were partly due to stimulating skeleton formation, inducing the production of some local factors of bone metabolism and decreasing the bone turnover rate.

关 键 词：老年性骨质疏松 快速老化小鼠P6 针刺 护骨素 骨形态发生蛋白-2 

分 类 号：R245[医药卫生—针灸推拿学]