栽培大麦染色体结构变异及其电脑图象自动分析  

IDENTIFICATION OF CHROMOSOMAL CHANGES IN BARLEY (HORDEUM VULGARE) USING A COMPUTER SYSTEM

作　　者：李浩兵[1,2] 仲裕泉 吕玉琦[1,2] 钟少斌 张德玉[1,2] 张旭[1,2] 姚景侠 

机构地区：[1]江苏省农业科学院农业生物遗传生理研究所 [2]东南大学生物医学工程系

出　　处：《江苏农学院学报》1997年第4期45-48,共4页Jiangsu Agricultural Research

基　　金：农业部资助;国家自然科学基金

摘　　要：以栽培大麦与球茎大麦种间杂种回交后代的２个抗病姐妹株（ＢＣ１－２、ＢＣ１－５）为材料，以其母本栽培大麦苏啤１号为对照，对它们的Ｃ－带染色体图象进行电脑技术处理：建立母本标准模式图，检测并绘制２个姐妹株的Ｃ－带染色体图象。结果表明，ＢＣ１－２Ｆ３的Ｃ－带带型与其母本基本相同，而ＢＣ１－５Ｆ３中有两对栽培大麦染色体发生了结构变异，其第三、四染色体长臂发生了互换，形成３／４易位。这种易位是栽培大麦染色体组间，而不是异源染色体间的易位。通过杂种早代花粉母细胞联会四价体Ｃ－带带型的电脑分析，进一步验证了这一结果。A C banding idiogram of barley cultivar “Su Pie” was established by computer, and used as a standard to analyze the C banded chromosomes of two barley lines BC 1-2 F 3 and BC 1 5 F 3, which derived from BC 1 of “Su Pie” X GBC141 (Hordeum bulbosum) The results showed that BC 1 2 F 3 had similar banding patterns to those of “Su Pie”, while a recipocal translocation occurred between chromosome 3 amd 4 of BC 1 5 F 3 with the long arms exchanged The formatiom of a quadrivalent in the meiotic cells of BC 1 5 F 1 further comfirmed the chromosome translocations detected by the computer system

关 键 词：大麦 易位系 品种 栽培品种 染色体 

分 类 号：S512.303.2[农业科学—作物学]