金鱼垂体细胞谱系基因切除研究  

GENETIC ABLATION OF CELL LINEAGE IN DENOHYPOPHYSIS OF GOLDFISH(CARASIUS AURATUS)

作　　者：常华[1] 沈玉[1] 王为先[1] 于建康 余来宁[2] 

机构地区：[1]中国科学院发育生物学研究所 [2]中国水产科学研究院长江水产研究所

出　　处：《中国水产科学》1997年第4期8-12,共5页Journal of Fishery Sciences of China

基　　金：国家自然科学基金

摘　　要：将大鳞大麻哈鱼的垂体细胞特异性表达的生长激素基因（ＧＨ）的启动子与白喉毒素基因的Ａ链相连，构建成融合基因ＧＨ－ＤＴＡ。通过显微注射导入金鱼（Ｃａｒａｓｉｕｓａｕｒａｔｕｓ）受精卵内，获得的实验鱼经Ｓｏｕｔｈｅｒｎｂｌｏｔ分子杂交检测表明，少数实验鱼染色体ＤＮＡ中含有导入的外源基因。胚胎的原位分子杂交和垂体的组织切片观察发现，转基因鱼和正常鱼之间存在差异。A hybrid gene GH-DTA, constructed with the promoter of Growth Hormone (GH) from Oncorhynchus tschawytscha and the “A” chain of diphtheria toxin, was introduced into the fertilized eggs of goldfish (Carrasius auratus) by microinjection. Southern blot and hybridization in situ were done to analyze the genomic DNA from the experimental goldfish. The results showed that GH-DTA was integrated into the chromosomal DNA of some goldfishes. Four adenohypohysis of the transgenic fish were sectioned and dyed with homotoxylin-eosin. It showed that the structure of the meso- adenohypophysis of the transgenic fish was different from that of the negative control goldfish..

关 键 词：转基因鱼 基因切除 金鱼 生物技术 

分 类 号：S965.8[农业科学—水产养殖]