人工合成锰(Ⅱ)-天冬氨酸SOD模拟物的毒性检测  

Toxicity Test of Mn^(2+)-Aspartat SOD Simulacrum

作　　者：薄芯[1] 李京霞[1] 

机构地区：[1]北京联合大学师范学院应用生物技术系,北京100011

出　　处：《药学进展》2008年第7期319-322,共4页Progress in Pharmaceutical Sciences

摘　　要：目的:对人工合成的SOD模拟物——锰(Ⅱ)-天冬氨酸进行初步毒理检测。方法:通过灌胃造成急性毒性确定半数致死量(LD50),并对小鼠亚急性毒性、骨髓嗜多染红细胞(PCE)微核试验进行了研究。结果:受试物对小鼠的LD50大于5 000 mg/kg,属于无毒。以亚急性毒性剂量500 mg/kg连续灌胃给药8天后,心、肝、脾、肺、肾和肠胃等器官在形状、大小、颜色等指标上与阴性对照组无明显差异。分别以500、1 500、2 500 mg/kg的剂量给小鼠连续灌胃两天,低剂量组的PCE微核率与阴性对照组比较无显著性差异,中剂量组与阴性对照组有显著性差异(P<0.05),高剂量组与阴性对照组有极显著性差异(P<0.01)。结论:锰(Ⅱ)-天冬氨酸属于微毒性,在大剂量使用时可导致一定程度的染色体损伤,但可在适当的剂量范围内控制使用。Objective： To examine the toxicity of Mn^2＋- Aspartat （ Mn^2＋-Asn）. Methods： The half lethal dose （LD50） was determined by acute toxicity test, and subacute toxicity and polychromatic erythrocyte （PCE） micronucleus tests in mice were conducted. Results： LD50 was found to be more than 5 000 mg/kg by oral administration. After stomach dosing injected at 500 mg/kg once daily, eight days in all consecutively, it showed no obvious influence on the weight of mice and the organs, such as heart, liver, spleen, lungs, kidney, intestines and stomach. After stomach dosing injected at 500, 1 500 and 2 500 mg/kg once daily, two days in all, the PCE micronucleus rates of mice were （ 1.9±0.74） ‰, （ 3.0±1.05） ‰ and （3.3 ±1.06） ‰, respectively. The lower dosage group showed no notable discrepancy from the normal mice （P 〉 0. 05 ）, but the middle and high dosages can increase the micronucleus rate （P 〈0.05 and P 〈0.01, respectively）. Conclusion： Mn^2＋- Asn in proper dosage can be used in the field of foods and medicines, but maybe it can damage chromosomes at high dosage.

关 键 词：锰(Ⅱ)-天冬氨酸 半数致死量 亚急性毒性 微核率 

分 类 号：R96[医药卫生—药理学]