美国月桂樱的组织培养及植株再生  

Tissue culture and plant regeneration of Laurocerasus caroliniana Ait.

作　　者：宿静[1] 汤庚国[1] 王光萍[1] 刘雅帅[1] 

机构地区：[1]南京林业大学森林资源与环境学院,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2008年第4期98-100,共3页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：镇江市科技计划项目(NY2007044)

摘　　要：以春季萌发的美国月桂樱嫩枝为外植体诱导丛生芽，在MS＋0．5mg／L BA＋0．01mg／L NAA的培养基上，其丛生芽诱导率达100％，在继代培养基中选择MS＋1．0mg／L BA＋0．1mg／L NAA培养基，不定芽增殖较快，后将增殖苗接入MS＋1．0mg／L KT＋0．1mg／L NAA培养基上壮苗，其生长状态最好。结果发现，赤霉素对芽的伸长没有效果。当芽伸长至3—4cm时，切下置于1／2MS＋1．0mg／L NAA的生根培养基中，生根率达100％，移栽成活率达40％。The faseieulated shoots were induced by the explant achieved from the juvenile branches of Laurocerasus caroliniana Ait. in spring. The inducing rate of fasciculated shoots attained 100% when they were cultured on the inducing medium of MS with BA 0. 5 mg/L and NAA 0.01 mg/L. The adventitious shoots were propagated most rapidly when subcuhured on the medium of MS with BA 1.0 mg/L and NAA 0.1 mg/L. Moreover, the adventitious shoots with best quality were obtained when they were cultured in the medium of MS with KT 1.0 mg/L and NAA 0.1 mg/L. The results of this experiment also indicated that gibberellin had no effects on the elongation of adventitious shoots. When the 3-4 cm adventitious shoots were cut and rooted in the rooting medium of 1/2 MS with NAA 1.0 mg/L, the rooting rate and surviving rate attained 100% and 40% respectively.

关 键 词：美国月桂樱 组织培养 植株再生 

分 类 号：S722.3[农业科学—林木遗传育种]