普通小麦与无融合生殖披碱草(Elymus rectisetus)属间杂种F_1的产生及其分子鉴定  

Production of Intergeneric Hybrid between Wheat and Apomictic Elymus rectisetus and Its Molecular Identification

作　　者：孙其信[1] 高建伟[1] 倪中福[1] 刘志勇[1] 

机构地区：[1]中国农业大学植物遗传育种系

出　　处：《农业生物技术学报》1997年第4期313-317,共5页Journal of Agricultural Biotechnology

摘　　要：本研究以普通小麦（ＴｒｉｔｉｃｕｍａｅｓｔｉｖｕｍＬ．；２ｎ＝６ｘ＝４２）品种Ｆｕｋｕｈｏｋｏｍｕｇｉ为母本，以无融合生殖披碱草（Ｅ．ｒｅｃｔｉｓｅｔｕｓ（ＮｅｓｉｎＬｅｈｍ）Ａ．ＬｏｖｅｅｔＣｏｎｎｏｒ；２ｎ＝６ｘ＝４２）品系１０５０为父本进行杂交，利用胚拯救技术，对杂种幼胚进行愈伤组织诱导、植株再生，获得了生长正常的属间杂种Ｆ１。对所获杂种在幼苗期用ＲＡＰＤ标记进行了鉴定，结果表明，有２１个引物的扩增产物Ｆ１呈现共显性，占扩增引物总数的２０．５９％；有６１个引物的扩增产物Ｆ１偏向父本，占扩增引物总数的５９．８０％；有２０个引物的扩增产物Ｆ１偏向母本，占扩增引物总数的１９．６１％。这表明杂种Ｆ１为真杂种。这一属间杂种的获得为进一步开展Ｅ．ｒｅｃｔｉｓｅｔｕｓ无融合生殖基因向小麦转移奠定了基础。本研究结果证明，ＲＡＰＤ标记可作为一种小麦属间杂种快速、有效、简便的分子鉴定方法。Elymus rectisetus((Nees in Lehm)A Love et Corman) is the only apomictic species found in the Triticeae.The intergeneric hybrid between wheat and the apomictic E.rectisetus accession 1050 was obtained through sexual hybridization followed by embryo rescue. Genomic DNAs was isolated from seedlings of the putative intergeneric hybrid and parents,and was used for RAPD analysis.Among the 105 primers,it was found the 102 primers show polymorphism between the parents.The amplification products from 21 primers show codominant,those of 20 primers are maternallike,while those of 61 primers are the same as male parent.This indicates that the hybrid we obtained is true hybrid,which will be useful for the further study of transfering the apomixis from E. rectisetus into wheat.

关 键 词：小麦 无融合生殖 披碱草 属间杂交 

分 类 号：S512.103.5[农业科学—作物学]