不同来源的棉花细胞在悬浮培养条件下诱导形成纤维的研究  

Studies on the Formation of Fibers in Cell Suspension Cultures Derived from Various Cotton (Gossypium hirsutum L.) Explants

作　　者：杨佑明[1] 李华恩 徐楚年 贾君镇 周海鹰 翟志席 

机构地区：[1]中国农业大学农学部,北京市农工商总公司信息中心,中国农业大学基础科技学院

出　　处：《农业生物技术学报》1997年第4期353-359,共7页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金;国家攀登计划资助

摘　　要：对源自棉花胚珠的外珠被、内珠被、珠心、以及下胚轴和多次继代培养的胚珠愈伤组织的细胞在悬浮培养条件下诱导纤维发育的能力进行了研究，结果表明：这五种来源的细胞均被诱导而分化形成纤维，说明棉花的非胚珠表皮细胞也具有分化形成纤维的能力。研究中还观察到游离的培养细胞表现出多种伸长方式。比较愈伤组织细胞诱导形成纤维的能力，内珠被和外珠被来源的细胞无明显差异，但二者明显高于下胚轴、珠心来源的细胞、以及多次继代培养的胚珠愈伤组织细胞。The ability to form fibers of cells in suspension cultures derived from the outer integuments, inner integuments,nucelluses, hypocotyles and ovulederived calli of cotton ( Gossypium hirsutum L.cv.Zhongmiansuo12 ) was studied.The results showed cells derived from these 5 kinds of explants could be induced to form fibers,suggesting nonepidemical cells of ovules could form fibers.The varied elongation modes of single cells in suspension cultures were also observed.The cells derived from various explants showed different ability to form fibers. The ability to form fibers of cells derived from inner and outer integuments was obviously higher than this of cells derived from nucelluses,hypocotyles and ovulederived calli after 300 days of continuing cultures,but the ability to form fibers of cells derived from inner integuments were insignificantly different from this of cells derived from outer integuments.

关 键 词：棉花 细胞悬浮培养 纤维形成 

分 类 号：S562.035.3[农业科学—作物学]