干旱胁迫下油菜素内酯对文冠果苗木抗氧化酶活性和抗氧化剂含量的影响  

Effects of brassinolide on antioxidant enzymes activities and antioxidants contents of Xanthoceras sorbifolia Bunde seedlings under drought stress

作　　者：冯朝红[1] 李凯荣[2] 张鹏文 刘建利[1] 

机构地区：[1]西北农林科技大学资环学院,陕西杨凌712100 [2]中国科学院水利部西北农林科技大学水土保持研究所黄土高原土壤侵蚀与旱地农业国家重点实验室,陕西杨凌712100 [3]内蒙古水利水电勘测设计院规划处,内蒙古呼和浩特010020

出　　处：《干旱地区农业研究》2008年第4期152-155,共4页Agricultural Research in the Arid Areas

基　　金：国家重点实验室基金(10501-163)

摘　　要：用蘸根和叶面喷施不同浓度油菜素内酯（BRs）的方法,在人工控制土壤水分条件下,对黄土高原重要造林树种文冠果苗木重要的抗氧化酶活性和抗氧化剂含量变化进行研究。结果表明,用0.05-0.4 mg/L BRs处理文冠果苗木,超氧化物歧化酶（SOD）活性在中度干旱胁迫下较清水对照增加,但差异不显著,重度胁迫下增加显著;在中度和重度胁迫下,各处理文冠果苗木过氧化氢酶（CAT）、过氧化物酶（POD）和抗坏血酸过氧化物酶（APX）活性均较清水对照升高,且差异显著;在中度和重度胁迫下,5个浓度BRs处理对抗坏血酸（ASA）和还原型谷光甘肽（GSH）含量均具显著的增加效应。轻度和重度胁迫下,0.2 mg/L BRs处理对文冠果苗木抗氧化酶活性和抗氧化剂含量的增加效应均最为显著。Important antioxidant enzymes activities and antioxidants contents of Xanthoceras sorbifolia Bunde seedlings were detected and analyzed by dipping roots and spraying leaves of seedlings with different concentrations of brassinolide under controlled soil water condition.The results showed that after treatment of 0.05～0.4 mg/L brassinolide,the activities of SOD of Xanthoceras sorbifolia Bunde seedlings under modest drought stress（soil moisture 11%～12%） and severe drought stress（soil moisture 7%～8%） were higher more than those untreated ones but with no significant difference under modest stress.The activities of CAT,POD and APX of Xanthoceras sorbifolia Bunde seedlings after treatment of 0.05～0.4 mg/L brassinolide were significantly higher than those of the untreated ones under modest drought stress and severe drought stress.The GSH and ASA contents of Xanthoceras sorbifolia Bunde seedlings after treatment of 0.05～0.4 mg/L brassinolide were significantly increased than the untreated ones.

关 键 词：干旱胁迫 抗氧化酶活性 抗氧化剂含量 油菜素内酯 文冠果 

分 类 号：S792.99[农业科学—林木遗传育种]