青蒿内生菌的分离及抗病活性物质的筛选  

Isolation and Screening of Endophytes from Artemisia annua and Their Antagonistic Activity to Plant Pathogens

作　　者：田小曼[1] 吴云锋[1] 张珏[1] 

机构地区：[1]西北农林科技大学植物保护学院与陕西省农业分子生物学重点实验室

出　　处：《西北农业学报》2008年第4期186-190,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金资助项目(30571214);教育部长江学者和创新团队发展计划资助(No.200558)

摘　　要：以菊科植物青蒿的根、茎、叶为材料,从中分离出内生菌63株,其中细菌43株,真菌12株,放线菌8株;以棉花枯萎、小麦赤霉、番茄叶霉等12种病原真菌做为靶标菌,研究内生菌的抗菌活性,筛选出了有较高抗菌活性的1株内生真菌、9株内生细菌和3株内生放线菌。Plant endophytic is a kind of microorganisms which spends all or most life in plant hosts and does not make any diseased symptom. In this research, the Artemisia annus＇s roots, stems and leaves were used as the material, initially separated 63 endophytic strains, including 43 bacterial strains, 12 fungal strains and 8 actinomycete strains. Using 12 kind of pathogenic fungi, such as Fusarium graminearurn, Fusariurn oa：ysporurn and Fulria fulv as the target, 1 fungal strains, 9 bacterial strains of fermentation fluid and 3 strains of actinomycete fermentation fluid were screened, which had the high antibacterial activities.

关 键 词：内生菌 青蒿 分离 活性筛选 

分 类 号：S567.219[农业科学—中草药栽培]