组培微环境对葡萄风信子组培苗生长及移栽后生理特性的影响  被引量:10

Effects of in Vitro Microenvironment on the Growth and Physiological Characteristics of Hyacinth Plantlets

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作  者:孙冬青[1] 王飞[1] 李新凤[1] 

机构地区:[1]西北农林科技大学园艺学院,陕西杨凌712100

出  处:《西北农业学报》2008年第4期244-248,262,共6页Acta Agriculturae Boreali-occidentalis Sinica

基  金:陕西省科技攻关项目"花卉新品种引进与示范"(2002ZK03-G7-4)

摘  要:研究了组培微环境对葡萄风信子组培苗生根阶段的苗生长及移栽后生理特性的影响。结果表明,生根培养时,光强和蔗糖对植株生长均有很大的影响,无论是有糖还是无糖培养,高光强均有利于植株干物质的积累,其中添加3%蔗糖有利于根的生长,并对根系活力和酶的活性有一定的促进作用,无糖处理有利于株高生长。移栽后,微环境调控对移栽成活率有一定的影响,有糖高光照处理的植株移栽后环境适应能力较强,存活率高,生长较好。The influence of in vitro microenvironment on the growth and physiology of hyacinth plantlets during rooting and naturalizing stages was studied. The results indicated that light intensity and sucrose have obvious influence on the plants growth during rooting stage. Higher light intensity was beneficial to the accumulation of solids,either with sucrose or free-sucrose. 30/00 sucrose was beneficial to the growth of plantlets' roots, 3% sucrose raised the activity of roots and antioxidases ,but freesucrose is beneficial to the growth of plantlets'height . On naturalizing stage , in vitro microenvironment has a certain degree influence on survival after plantlets transfer. Plantlets with 3% sucrose and higher light intensity had the stronger ability to accommodate to circumstances, had the highest survival rate and better growth expression after transfer.

关 键 词:风信子 组织培养 微环境 移栽 

分 类 号:S682.29[农业科学—观赏园艺]

 

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