促红细胞生成素和人生长激素兴奋剂检测方法的研究进展  

Recent advances in the detection of human erythropoietin and human growth hormone doping

作　　者：郭磊[1] 张朝阳[1] 唐吉军[1] 谢剑炜[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《色谱》2008年第4期437-443,共7页Chinese Journal of Chromatography

基　　金：国家自然科学基金资助项目(批准号:20705039)

摘　　要：在2008年世界反兴奋剂组织颁布的兴奋剂目录中,S2项肽类激素及相关品种均属于内源性生物大分子物质,如何区分所检测物质属于外界摄入还是机体分泌是此项检测的重点与难点。本文针对其中应用最为普遍、研究较为深入的促红细胞生成素(EPO)和人生长激素(hGH)的检测,从间接血液指标检测、直接检测途径等方面进行了分类评述,侧重于从理化分析方法、免疫分析方法角度阐述识别及区分重组蛋白与内源性蛋白的新途径。In the World Anti-doping Agency 2008 Prohibited List, the prohibited substances of S2 item are hormones and related substances, which are belonging to the endogenous biomacromolecules. How to identify the substances derived from endogenous secretion or exogenous administration is the main problem in doping control analysis and attracts more attention. The present report summarizes the main analytical strategies, including indirect blood tests and direct detection approaches developed to identify the presence of erythropoietin （ EPO ） and human growth hormone （hGH）, which have wide pharmaceutical applications and thus been fully examined. The recent physico-chemical or immunoanalytical methodologies on the discrimination of recombinant and endogenous proteins are emphasized.

关 键 词：内源性物质 促红细胞生成素 人生长激素 兴奋剂检测方法 进展 

分 类 号：O658[理学—分析化学]