毛细管电泳应用于测定牛血清白蛋白与脂质体的相互作用  

Investigation on interaction between bovine serum albumin and liposome using capillary electrophoresis

作　　者：厉红[1] 屈锋[1] 徐建栋[1] 邓玉林[1] 

机构地区：[1]北京理工大学生命科学与技术学院,北京100081

出　　处：《色谱》2008年第4期473-477,共5页Chinese Journal of Chromatography

基　　金：国家自然科学基金资助项目(No.20275005)

摘　　要：建立了一种用毛细管电泳法检测牛血清白蛋白（BSA）与脂质体相互作用的分析方法。氧化指数的测定实验结果表明经过冷冻干燥的脂质体稳定性更好；毛细管电泳表征脂质体的电荷性质实验结果表明脂质体在pH5．0—8．0的条件下呈电中性。在pH7．0的条件下，以各种浓度的脂质体混悬液为电泳缓冲液，以0．8％二甲亚砜（DMSO）为内标，随着缓冲液中脂质体质量浓度从0增加到2．4mg／mL，BSA的有效淌度从-2．232×10^-4cm^2·V^-1·s^-1变化到-3．046×10^-4cm^2·V^-1·s^-1结合Scatchard分析，测得BSA与脂质体的结合常数为2．522×10^3（g／mL）^-1。该方法简单、快速，为研究蛋白质与脂质体的相互作用提供了新的技术手段。A method for the investigation on the interaction between bovine serum albumin （BSA） and liposome using capillary electrophoresis was developed. The oxidation index showed that the hposomes after freeze-drying were more stable. The results obtained from the capillary electrophoretic analysis of liposome showed that liposome had no charge at pH 5.0 - 8.0. A series of liposome suspension at different concentrations with the internal marker of 0. 8% dimethyl sulfoxide （ DMSO ） were introduced as the electrophoresis buffer at pH 7.0. Along with the liposome concentrations raised from 0 to 2.4 mg/mL, it was found that the effective mobility of BSA changed from -2. 232 × 10-4 cm^2 · V^-1· s^-1 to -3. 046× 10-4 cm^2 · V^-1· s^-1 The binding constant between BSA and liposome was 2. 522 × 103（g/mL） ^-1 calculated by Scatchard analysis. This method is simple and rapid, and provides a new technology for the investigation on the interactions between protein and liposome.

关 键 词：毛细管电泳 牛血清白蛋白 脂质体 

分 类 号：O658[理学—分析化学]