高效液相色谱法同时测定罗汉果中的六种葫芦烷三萜类皂苷  

Simultaneous determination of six cucurbitane triterpene glycosides in Siraitia grosvenorii fruits using high performance liquid chromatography

作　　者：卢凤来[1] 刘金磊[1] 黄永林[1] 李典鹏[1] 

机构地区：[1]广西壮族自治区中国科学院广西植物研究所,广西桂林541006

出　　处：《色谱》2008年第4期504-508,共5页Chinese Journal of Chromatography

基　　金：中国科学院"西部之光"人才培养计划入选项目(科发人教字[2002]101号);桂林市科技经费资助项目(编号:20070304-2)

摘　　要：建立了高效液相色谱测定罗汉果中罗汉果苷Ⅴ、罗汉果苷ⅣA、罗汉果苷Ⅲ、11-氧化罗汉果苷Ⅲ、罗汉果苷ⅡE和11-氧化罗汉果苷ⅡE等6种苷类化合物含量的方法。采用的色谱条件:ZORBAX SB-C18色谱柱(150mm×4.6mm,5μm);柱温30℃;以水(A相)和乙腈(B相)为流动相,梯度洗脱程序:0~3min,20%B^30%B;3~8min,30%B^35%B;8~9min,35%B;流速0.8mL/min;检测波长203nm;进样量10μL。结果表明,以上6种苷类化合物分别在0.04~1.0mg/mL、0.011~0.68mg/mL、0.010~0.80mg/mL、0.009 7~0.58mg/mL、0.025~1.0mg/mL、0.013~0.76mg/mL范围内线性关系良好(r>0.999 1)。加标回收率依次为99.65%,101.6%,97.05%,103.1%,99.25%和103.0%;相对标准偏差分别为0.83%,3.1%,1.9%,3.3%,0.59%和2.0%。该方法简便、快速、准确,适用于罗汉果原材料及其制品中苷类化合物的检测和含量测定。Siraitia grosvenorii, a traditional Chinese fruit, belongs to the family Cucurbitaceae and has been used as a pulmonary demulcent and emoilient for the treatment of dry cough, sore throat, dire thirst, and constipation in folk medicine. A high performance liquid chromatographic method was developed for simultaneous determining the contents of mogroside Ⅴ, mogrosideⅣA, mogroside Ⅲ, 11-oxomogroside Ⅲ, mogroside Ⅱ E and 11-oxomogroside Ⅱ E in Siraitia grosvenorii fruits. The chromatographic analysis was carried out on a ZORBAX SB-Cl8 column （150 mm ×4.6 mm, 5 μm）. The mobile phase was water （A） and acetonitrile （B） with gradient elution （0 -3 min, 20% B -30% B; 3 -8 min, 30% B -35% B; 8 -9 min, 35% B）. The flow rate was maintained at 0. 8 mL/min. The detection wavelength was set at 203 nm and the column temperature was controlled at 30 ℃. The sample injection volume was 10 μL. The calibration curves were linear over the ranges of 0.04 - 1.0 mg/mL, 0.011 - 0.68 mg/mL, 0.010 -0.80 mg/mL, 0. 009 7 -0.58 mg/mL, 0. 025 - 1.0 mg/mL and 0. 013 -0.76 mg/mL （r〉 0. 999 1 ） for the above cucurbitane triterpene glycosides, respectively. The average recoveries were 99.65% for mogrosideV, 101.6% for mogroside ⅣA, 97.05% for mogroside 111, 103. 1% for 11-oxomogroside Ⅲ, 99.25% for mogroside Ⅱ E, and 103.0% for 11-oxomogroside Ⅱ E, with the relative standard deviations of 0.83%, 3. 1%, 1.9%, 3.3%, 0.59% and 2.0%, respectively. This simple, rapid and accurate method is suitable for quality control and determination of raw materials and products of Siraitia grosvenorii fruits.

关 键 词：高效液相色谱法 葫芦烷三萜类皂苷 罗汉果 

分 类 号：O658[理学—分析化学]