薄层色谱结合凝胶成像系统测定小鼠表皮神经酰胺含量  

Determination of Ceramide in Mouse Epiderm by Thin-layer Chromatography and G el Image-Forming System

作　　者：王妍[1] 林青[1] 谢金梅 何丹[1] 李钦[1] 

机构地区：[1]云南中医学院药理教研室,昆明650200

出　　处：《中药新药与临床药理》2008年第4期281-282,共2页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：云南省教育厅科学研究基金(06J352C)

摘　　要：目的建立皮肤表皮中神经酰胺含量的测定方法。方法抽提动物表皮脂质,采用薄层层析、BIO-RAD GelDoc凝胶成像系统采集图像、Quantity One软件分析测定神经酰胺含量。结果神经酰胺在0.416～2.08μg范围内,含量与密度积分值有良好线性关系,相关系数r=0.9953;同板精密度良好,RSD=1.09%;异板精密度良好,RSD=2.10%;对照品溶液与供试品溶液在2h内显色稳定,重现性良好,RSD=3.03%;平均加样回收率为99.58%,RSD=1.46%;样品测定结果RSD=3.10%。结论该法可用于皮肤表皮中神经酰胺的含量测定。Objective To establish a method for determining the content of ceramide in skin epidermis. Methods Epidermal lipids of mice were extracted by routine method, separated by thin - layer chromatography （TLC）, and shot by BIO - RAD Gel- Doc system. Epidermal ceramide content was determined by Software of Quantity One. Results A good linearity was obtained in the range of 0. 416 - 2.08 μg （ r = 0. 995 3）. The precision of the same plate was good, RSD = 1.09 % ; and the precision of different plates was also good, RSD = 2. 10 %. The stability of control solution and sample solution was good within 2 b. The reproducibility was good, RSD = 3.03 % , and the mean recovery rate was 99.58 % and RSD = 1.46 %. The RSD of simple determination was 3.10 %. Conclusion This method can be used to determine the content of ceramide in skin epidermis.

关 键 词：神经酰胺 含量测定 薄层层析 凝胶成像 

分 类 号：R285.5[医药卫生—中药学]