整合蛋白β1亚基过表达上调p27抑制肝癌细胞SMMC-7721的增殖(英文)  

Integrin β1 overexpression inhibits cell proliferation by upregulating p27 in human hepatocellular carcinoma cell line SMMC-7721

作　　者：傅奕[1] 梁玉龙[2] 

机构地区：[1]扬州大学医学院生化教研室,江苏扬州225001 [2]美国贝勒医学院生物化学与分子生物学系,休斯敦77030

出　　处：《江苏大学学报（医学版）》2008年第4期281-286,290,共7页Journal of Jiangsu University：Medicine Edition

基　　金：This work was supported by The National Natural Science Foundation of China(30570963).

摘　　要：目的:研究整合蛋白β1亚基过表达对肝癌细胞SMMC-7721增殖的影响及机制。方法:采用MTT及细胞流式测定观察整合蛋白β1亚基过表达对肝癌细胞SMMC-7721增殖及细胞周期的影响。蛋白质印迹及RT-PCR检测细胞中p27的蛋白及mRNA的表达,并构建p27的RNA干扰质粒,转染整合蛋白β1亚基过表达细胞,再观察p27的表达变化对细胞增殖的影响。结果:整合蛋白β1亚基过表达可以抑制肝癌细胞SMMC-7721的增殖,并将细胞阻滞在细胞周期的G1期。整合蛋白β1亚基过表达可以在蛋白水平上调p27的表达,但并不影响p27的mRNA水平。p27的RNA干扰质粒可明显抑制p27的表达,而且p27表达的下调可以阻止整合蛋白β1亚基过表达所引起的细胞增殖抑制。结论:整合蛋白β1亚基过表达通过上调p27抑制肝癌细胞SMMC-7721的增殖。Objective： To investigate the mechanism of integrin 61 overexpression regulating cell proliferation of hepatocellular carcinoma cell line SMMC-7721. Methods： MTT and FACS assay were used to analyz the influence of integrin 61 overexpression on cell proliferation and cell cycle. The protein and mRNA expression of p27 were measured by Western blot and RT-PCR. RNAi approach was employed to silence the expression of p27. Then this plasmid（SiRNA-p27） was transfected into integrin 61 overexpressing cells, and cell growth was analyzed. Results： Overexpression of integrin 61 inhibited cell growth in SMMC-7721 cells and induced G1 phase arrest. Integrin 61 overexpression increased the protein level of p27, but not mRNA level, siRNA-p27 plasmid effectively silenced the expression of p27, and knock-down of p27 blocked the cell growth inhibition induced by integrin 61 overexpression. Conclusion： Overexpression of integrin 61 inhibited cell proliferation by upregulating p27 in human hepatocellular carcinoma cell line SMMC-7721.

关 键 词：整合蛋白 过表达 P27 细胞增殖 RNA干扰 

分 类 号：Q55[生物学—生物化学] R72[医药卫生—儿科]