抗原负载的DC和CIK共培养后对MGC-803的杀伤活性  

Studies on the lytic activity of cytokine-induced killer cells against MGC-803 after co-culture with antigen-pulsed dendritic cells

作　　者：杨葳[1] 吴小娥[2] 王云虹[3] 高锦[3] 徐铭宝[2] 

机构地区：[1]武警医学院,天津300162 [2]武警总医院干部二科,北京100039 [3]中科院生物物理研究所,北京100101

出　　处：《武警医学院学报》2008年第9期727-730,734,共5页Acta Academiae Medicinae CPAPF

摘　　要：【目的】研究负载抗原后的树突状细胞(DC)对细胞因子诱导的杀伤细胞(CIK)增殖活性、表型和功能的影响。【方法】分离外周血单个核细胞(PBMC),经不同细胞因子作用后定向分化成DC和CIK细胞,以胃癌细胞株MGC-803冻融物作为肿瘤抗原刺激DC后与CIK共培养,以流式细胞仪检测CIK细胞膜表面分子表达,MTT法检测共培养后CIK细胞对MGC-803杀伤活性的变化。【结果】CIK与负载MGC-803抗原的DC共培养后3d增殖活性开始显著提高,最高增殖倍数可达1 179.5±1.29;CD3+CD8+和CD3+CD56+细胞较共培养前增多,且与负载抗原的DC共培养的CIK具有比单独培养的CIK更强的杀瘤活性。【结论】以肿瘤细胞冻融物诱导成熟的DC能进一步促进CIK增殖,提高其对胃癌细胞的杀伤活性,为指导临床应用DC和CIK联合治疗消化道肿瘤提供了实验依据。[Objective] To study the effect of antigen-pulsed dendritic cells （DC） on the proliferation, phenotype and function of cytokine-induced killers （CIK） . [Methods] Gastric cell line MGC-803 lysates were used as antigen and pulse DC with the lysates. The expression of the molecules on the surface of CIK cell membrane is tested by FCM and the cytotoxicity of CIK was determined by MTT after co-culture with antigen-pulsed DC. [Results] The activity of proliferation of CIK began to strengthen on the third day after co-culture with antigen-pulsed DC, with the peak of 1 179.5 ± 1.29 times. The number of CD3^＋ CD8^＋ and CD3^＋ CD56^＋ cells increased and the lytic activity of CIK strengthened as well. [Conclusions] Tumor-lysate-pulsed DC can further strengthen the proliferation and lytic activity of CIK against gastric cells, which will provide experimental evidence to clinical treatment of enteron tumor with the application of com- bination of DC and CIK.

关 键 词：树突状细胞 细胞因子诱导的杀伤细胞 MGC-803 胃癌 抗原 

分 类 号：R73[医药卫生—肿瘤]