金莲花不同花期的花粉活力研究  

Study on Pollen Viability of TroUius Chinensis in Different Flowering Phase

作　　者：朱殿龙[1,2] 李勇[1,2] 丁万隆[1,2] 陈士林[1,2] 

机构地区：[1]中国医学科学院 [2]中国协和医科大学药用植物研究所,北京100094

出　　处：《中国现代中药》2008年第7期22-23,共2页Modern Chinese Medicine

基　　金：国家科技攻关项目(2004BA721A16);国家科技支撑计划项目(2006BAI06A13-03)

摘　　要：目的:测定金莲花花粉活力。方法:采用离体萌发法对金莲花不同花期的花粉活力进行测定,配制不同浓度(5%,10%,15%,20%)的蔗糖溶液,接种不同花期的花粉,统计花粉萌发率。结果:金莲花花粉在供试不同浓度蔗糖溶液中均可萌发,在5%～10%蔗糖溶液中萌发率较高;始开期或盛开期金莲花花粉萌发率差异不大,花蕾期金莲花花粉萌发率极低。结论:5%～10%的蔗糖溶液适合进行金莲花花粉离体活力测定;始开期和盛开期金莲花花粉活力较花蕾期高。Objective： To study the pollen viability of Trollius chinensis. Methods： pollen germination method in vitro were used to study pollen viability of Trollius chinensis treated with sucrose solution in 4 different concentrations. Results： In this study, the optimum condition for Trollius chinensis pollen germination is 5% - 10% sucrose solution, pollen in the bud time can hardly sprout, the difference of pollen germination rate between earlier period and later time of florescence were not significant. Conclusion： 5% to 10% sucrose solution were fitted to study pollen viability of Trollius chinensis, and pollen germination rates of the earlier period and later time of florescence were signifi- cantly higher than the bud time.

关 键 词：金莲花 花粉活力 花粉萌发率 

分 类 号：R282.21[医药卫生—中药学] R282.71[医药卫生—中医学]