大鼠关节软骨细胞的培养  

CULTURE OF ARTICULAR CHONDROCYTES FROM RAT

作　　者：宋永周[1] 崔慧先[2] 吕哲[3] 李莎[2] 郭威[2] 

机构地区：[1]河北医科大学第二医院骨科,河北石家庄050000 [2]河北医科大学基础医学院解剖学教研室,河北石家庄050017 [3]河北医科大学第二医院耳鼻咽喉科,河北石家庄050000

出　　处：《河北医科大学学报》2008年第4期534-536,I0010,共4页Journal of Hebei Medical University

摘　　要：目的探讨大鼠关节软骨细胞培养方法。方法采用胶原酶NB4消化法从1周龄SD大鼠的关节软骨中分离出软骨细胞并进行原代和传代培养,每日在倒置显微镜下观察细胞形态及其生长情况,并用HE、甲苯胺蓝染色和Ⅱ型胶原免疫组织化学染色进行鉴定。结果软骨细胞形成单层的时间在原代培养1周左右,5d左右即可传代。第5代后部分变为梭形,第7代后绝大部分变为长梭形,增殖能力减弱。结论采用此法可在短时间内获得大量纯化的大鼠软骨细胞。Objective To study the method of isolation and culture of articular chondrocytes from rat. Methods Articular chondrocytes were obtained from the cartilage of SD rat aged 1 week digested by collagenase NB4. Primary culture and subculture of chondrocytes were conducted. The morphological changes and growth feature were recorded under the inverted microscope each day. The chondrocytes were biologically confirmed by HE staining, toluidine blue and immunocytochemical staining of type Ⅱ collagenase. Results The time for chondrocytes to develop a monolayer was one week in primary culture,while five days in subculture. Some of the cells of the 5th generation turned to spindle-shape,after the 7th generation,the cells became long spindle-shape. The ability of proliferation after 7th generation was weaker. Conclusion The method to isolate and culture of articular chondrocytes in SD rat can acquire pure and enough cartilage cells in short time.

关 键 词：软骨细胞 细胞培养技术 大鼠 

分 类 号：Q813.11[生物学—生物工程]